Description

12-o-Hexadecanoylphorbol-13-Acetate is a potent and specific protein kinase C (PKC) activator, widely recognized as a key biochemical tool in life science research. Its primary value lies in its ability to mimic the action of diacylglycerol (DAG), making it indispensable for studying PKC-mediated signal transduction pathways in cellular and molecular biology. This compound is essential for researchers and pharmaceutical developers in the fields of oncology, immunology, and dermatology, where precise modulation of PKC activity is critical for experimental models and drug discovery efforts.

Application

• Biochemical Research: As a standard PKC activator for studying enzyme kinetics, signal transduction mechanisms, and second messenger systems in vitro.
• Cell Biology Studies: Used to induce cellular responses such as proliferation, differentiation, and apoptosis in various cell lines to model disease states.
• Cancer Research: Employed in oncology research to investigate tumor promotion, PKC's role in carcinogenesis, and to screen for potential PKC-inhibiting therapeutics.
• Immunology & Inflammation Research: Applied to activate T-cells, neutrophils, and other immune cells to study inflammatory pathways and immune responses.
• Neuroscience: Utilized to examine the role of PKC in neuronal plasticity, neurotransmitter release, and long-term potentiation.
• Dermatological Research: Used in skin model studies to understand PKC's involvement in skin tumor promotion and other epidermal disorders.
• Pharmaceutical Development: Serves as a critical reference standard and pharmacological tool in the discovery and validation of novel PKC-targeted drugs.

Basic Information

Product Name	12-o-Hexadecanoylphorbol-13-Acetate
CAS No.	20839-12-7
Molecular Formula	C36H56O8
Molecular Weight	616.83 g/mol
Synonyms	TPA; Phorbol 12-myristate 13-acetate; PMA; Tetradecanoylphorbol Acetate; Phorbol 12,13-dimyristate; NSC 350836; 12-O-Tetradecanoylphorbol-13-acetate; 12-O-Tetradecanoylphorbol 13-acetate
EINECS	244-087-8

Quality Control

Our 12-o-Hexadecanoylphorbol-13-Acetate is produced and handled under strict quality management protocols to ensure the high purity and consistency required for sensitive research applications. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality parameters to support your research integrity and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, consider storing desiccated under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, ethanol; slightly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.