Description

Tlck CAS NO 2364-87-6 is a highly specific and potent synthetic inhibitor of trypsin-like serine proteases, including trypsin and thrombin. This compound is critical for biochemical research and pharmaceutical development where precise enzyme inhibition is required. It is primarily utilized by researchers in proteomics, structural biology, and drug discovery, as well as by manufacturers developing diagnostic kits and therapeutic agents targeting coagulation and inflammatory pathways.

Application

• Biochemical Research: As a specific active-site titrant and inhibitor for mechanistic studies of serine proteases like trypsin, thrombin, and plasmin.
• Pharmaceutical Development: Used in high-throughput screening (HTS) assays to identify and characterize novel protease inhibitors for therapeutic applications.
• Diagnostic Reagent Manufacturing: A key component in the formulation of control reagents and calibration standards for clinical diagnostic tests measuring coagulation factors.
• Proteomics & Cell Biology: Employed to selectively inhibit specific proteolytic activities in cell lysates and tissue samples to study protein function and signaling pathways.
• Structural Biology: Used to form stable enzyme-inhibitor complexes for X-ray crystallography and NMR studies to elucidate protease active site architecture.

Basic Information

Product Name	Tlck
CAS No.	2364-87-6
Molecular Formula	C14H22Cl2N4O2
Molecular Weight	349.26 g/mol
Synonyms	TLCK; Tosyl-L-lysyl-chloromethane hydrochloride; Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride; TLCK hydrochloride; L-1-Chloro-3-tosylamido-7-amino-2-heptanone hydrochloride; Nα-p-Tosyl-L-lysine chloromethyl ketone hydrochloride; Antipain (component); Protease Inhibitor TLCK
EINECS	219-124-5

Quality Control

Our Tlck is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, NMR for structural confirmation, and elemental analysis. A detailed Certificate of Analysis (COA) providing identity, purity, and impurity profiles is supplied with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, colorless solution at 10 mg/mL in DMSO or 1% Acetic Acid

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.