Description

6-Hydroxymelatonin is a primary metabolite of melatonin, formed via cytochrome P450-mediated hydroxylation. This compound is of significant interest as a key biomarker for melatonin metabolism in clinical and pharmacological research. It serves as a crucial reference standard and intermediate for researchers and manufacturers in the pharmaceutical, nutraceutical, and life science industries developing analytical methods and studying sleep-wake cycles, antioxidant pathways, and chronobiology.

Application

• Pharmaceutical Reference Standard: Used as a high-purity certified standard for the quantitative analysis of melatonin and its metabolites in biological fluids via HPLC, LC-MS, or GC-MS.
• Biochemical Research: Essential for in vitro and in vivo studies investigating melatonin's metabolic pathways, pharmacokinetics, and antioxidant mechanisms.
• Diagnostic Kit Development: A key component in the development of immunoassays (ELISA, RIA) for measuring melatonin metabolite levels in clinical diagnostics.
• Nutraceutical Analysis: Employed in quality control laboratories to assay melatonin content and purity in dietary supplements and finished products.
• Metabolite Synthesis: Acts as a critical synthetic intermediate for the preparation of other melatonin-related metabolites or labeled compounds (e.g., deuterated standards).
• Neuroscience & Chronobiology: Used in research models to understand the role of melatonin metabolism in sleep disorders, circadian rhythm regulation, and neuroprotection.

Basic Information

Product Name	6-Hydroxymelatonin
CAS No.	2208-41-5
Molecular Formula	C₁₃H₁₆N₂O₂
Molecular Weight	232.28 g/mol
Synonyms	6-Hydroxy-N-acetyl-5-methoxytryptamine; 6-OH-Melatonin; N-[2-(6-Hydroxy-5-methoxy-1H-indol-3-yl)ethyl]acetamide; Melatonin, 6-hydroxy-; 5-Methoxy-6-hydroxytryptamine acetate; 6-Hydroxymelatonin (primary metabolite)
EINECS	Contact for details

Quality Control

Our 6-Hydroxymelatonin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and assay, IR and NMR for structural identification, and stringent control of related substances. We provide full traceability and a Certificate of Analysis (COA) with each shipment, ensuring compliance with research and development grade specifications. Custom purity grades, including analytical reference standards, are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (e.g., nitrogen or argon) at controlled room temperature (15-25°C) or as specified on the label or COA. The material is light-sensitive and easily oxidized; prolonged exposure to air, light, or elevated temperatures should be avoided to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.