Description

Tosyllysine Chloromethyl Ketone CAS NO 2104-86-1 is a potent and selective irreversible inhibitor of trypsin-like serine proteases, specifically designed to covalently modify the active site histidine residue. This compound is a critical research tool for studying protease function, signal transduction pathways, and apoptosis. It is essential for biochemical research, pharmaceutical development, and proteomics applications where precise enzyme inhibition is required.

Application

• Biochemical Research: Used as a specific, irreversible inhibitor of trypsin, plasmin, thrombin, and other trypsin-like serine proteases to elucidate their biological roles.
• Pharmaceutical Development: Serves as a key reference standard and pharmacological tool in the discovery and validation of novel protease-targeted therapeutics.
• Proteomics & Cell Biology: Employed to inhibit specific proteases in cell lysates and complex biological samples to study proteolytic cascades and signaling pathways.
• Apoptosis Studies: Utilized to investigate the role of specific caspases and other proteases in programmed cell death mechanisms.
• Enzyme Characterization: Acts as an active-site titrant and mechanistic probe for determining the concentration and kinetic parameters of serine proteases.
• Diagnostic Reagent Manufacturing: Incorporated into the development of diagnostic kits and assays that require controlled protease inhibition.

Basic Information

Product Name	Tosyllysine Chloromethyl Ketone
CAS No.	2104-86-1
Molecular Formula	C17H25ClN2O4S
Molecular Weight	388.91 g/mol
Synonyms	TLCK; Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride; TLCK-HCl; Tosyl-L-lysyl-chloromethane hydrochloride; L-1-Chloro-3-tosylamido-7-amino-2-heptanone hydrochloride; Nα-p-Tosyl-L-lysine chloromethyl ketone hydrochloride; TPCK analog (for trypsin); Protease Inhibitor TLCK
EINECS	218-260-4

Quality Control

Every batch of Tosyllysine Chloromethyl Ketone is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure identity, purity, and performance. We provide full traceability and Certificates of Analysis (COA) are available for all shipments, detailing key parameters such as assay, optical rotation, and impurity profiles. Our quality commitment aligns with the standards expected for high-purity biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Optical Rotation	-15° to -18° (c=1 in H2O)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.1%
Biological Activity	Inhibits trypsin activity as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.