Description

Doxycycline-13C-D3 is a stable isotope-labeled analog of the broad-spectrum tetracycline antibiotic, doxycycline, specifically labeled with carbon-13 and deuterium. This compound is an essential internal standard for the precise and accurate quantification of doxycycline in complex biological matrices using advanced analytical techniques like LC-MS/MS. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in pharmacokinetic studies, drug metabolism research, and bioanalytical method development to ensure data integrity and regulatory compliance.

Application

• Bioanalytical Reference Standard: Serves as a critical internal standard for the quantification of doxycycline in plasma, serum, urine, and tissue samples via mass spectrometry.
• Pharmacokinetic & Metabolism Studies: Enables precise tracking of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Veterinary Drug Residue Analysis: Used in food safety testing to monitor and quantify doxycycline residues in meat, milk, and other animal-derived products.
• Environmental Fate Studies: Applied in research to trace the environmental persistence and degradation pathways of antibiotic compounds.
• Forensic & Doping Control: Supports accurate identification and quantification of doxycycline in forensic toxicology and anti-doping programs.
• Method Development & Validation: Essential for developing, optimizing, and validating robust LC-MS/MS methods in compliance with FDA, EMA, and ICH guidelines.

Basic Information

Product Name	Doxycycline-13C-D3
CAS No.	1902958-13-7
Molecular Formula	C22H21D3 13CN2O8
Molecular Weight	~467.45 g/mol
Synonyms	Doxycycline-(phenyl-13C6, 2',6'-D2); Doxycycline-13C6,D2; Doxycycline (13C6,D2); 4-(Dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-2-naphthacenecarboxamide-13C6,D2; (4S,4aR,5S,5aR,6R,12aS)-4-(Dimethylamino)-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-1,4,4a,5,5a,6,11,12a-octahydro-2-tetracenecarboxamide-13C6,D2; Stable Isotope Labeled Doxycycline
EINECS	Contact for details

Quality Control

Every batch of Doxycycline-13C-D3 is manufactured and analyzed under strict quality systems. We provide comprehensive analytical data to support your regulatory submissions, including Certificates of Analysis (COA) detailing isotopic purity, chemical purity, and identity confirmation via NMR, HPLC, and MS. Our quality commitment ensures the material meets the stringent requirements for use as a certified reference material in GLP/GMP-compliant environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	Yellow to light yellow powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity	≥99 atom % 13C; ≥98 atom % D
Chemical Purity	≥95%
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.