Description

Chir-99021 (Monohydrochloride) is a potent and selective small molecule inhibitor of glycogen synthase kinase-3 (GSK-3), widely recognized for its role in stem cell research and regenerative medicine. Its primary value lies in its ability to enhance the self-renewal and pluripotency of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), making it a critical reagent for advanced cell culture protocols. This high-purity compound is essential for researchers and developers in pharmaceutical R&D, biotechnology, and academic institutions focused on cell therapy, disease modeling, and drug discovery platforms.

Application

• Stem Cell Culture & Maintenance: Used as a key component in defined media formulations to maintain the undifferentiated state and pluripotency of human and mouse embryonic stem cells (ESCs).
• Induced Pluripotent Stem Cell (iPSC) Generation: Enhances the efficiency and quality of somatic cell reprogramming to a pluripotent state.
• Regenerative Medicine Research: Facilitates studies aimed at directing stem cell differentiation into specific lineages for tissue engineering and therapeutic applications.
• Disease Modeling & Drug Screening: Enables the creation of more robust and physiologically relevant in vitro disease models using patient-derived stem cells for high-throughput screening.
• GSK-3β Signaling Pathway Studies: Serves as a critical pharmacological tool for investigating the role of GSK-3 in Wnt/β-catenin signaling, metabolism, and neurological disorders.
• Biopharmaceutical Development: Supports process development for cell-based therapies by optimizing expansion protocols for therapeutic stem cell lines.

Basic Information

Product Name	Chir-99021 (Monohydrochloride)
CAS No.	1797989-42-4
Molecular Formula	C₂₂H₁₈ClN₃O • HCl
Molecular Weight	420.31 g/mol (monohydrochloride salt)
Synonyms	CHIR-99021; CHIR99021; CT99021; Laduviglusib; 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile hydrochloride; GSK-3 Inhibitor XV; GSK3 Inhibitor IX; CT-99021 HCl; 3-Pyridinecarbonitrile, 6-[[2-[[4-(2,4-dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-, hydrochloride (1:1)
EINECS	Contact for details

Quality Control

Our Chir-99021 (Monohydrochloride) is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive biological applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity determination and spectroscopic confirmation of structure. Certificates of Analysis (COA) detailing all specifications and test results are provided and available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (IR)	Spectrum matches reference
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.5%
Heavy Metals	≤20 ppm
Biological Activity (Cell-based assay)	IC₅₀ ≤ 10 nM for GSK-3β inhibition

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.