Description

L-Cysteine-15N,D3 is a stable isotope-labeled derivative of the essential amino acid L-Cysteine, specifically enriched with nitrogen-15 and deuterium. This compound is a critical tool for advanced research and analytical applications, providing a non-radioactive tracer for precise metabolic and pharmacokinetic studies. It is primarily utilized by pharmaceutical R&D teams, analytical laboratories, and academic researchers in the fields of proteomics, metabolomics, and drug discovery.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a quantitative reference standard in LC-MS and GC-MS analysis for accurate measurement of endogenous L-Cysteine levels in biological samples.
• Metabolic Pathway Tracing: Used in in vivo and in vitro studies to track the incorporation and fate of cysteine in glutathione synthesis, protein metabolism, and other biochemical pathways.
• Pharmaceutical Research & Development: Enables precise pharmacokinetic (PK) and absorption, distribution, metabolism, and excretion (ADME) studies for drug candidates targeting sulfur-containing amino acid pathways.
• Proteomics and Biomarker Discovery: Facilitates stable isotope labeling by amino acids in cell culture (SILAC) and other quantitative proteomic techniques for protein expression profiling.
• NMR Spectroscopy Studies: The 15N and 2H (D) labels provide distinct signals for structural and dynamic analysis of peptides and proteins using nuclear magnetic resonance spectroscopy.
• Nutritional and Clinical Research: Investigates cysteine metabolism, redox status, and its role in conditions related to oxidative stress.

Basic Information

Product Name	L-Cysteine-15N,D3
CAS No.	1795787-05-1
Molecular Formula	C3H4D315NO2S
Molecular Weight	124.17 g/mol (for 15N, D3)
Synonyms	(2R)-2-Amino-3-sulfanylpropanoic-15N,3,3,3-d3 acid; L-Cysteine-(15N, 3,3,3-d3); L-Cysteine-15N,d3; Cysteine-15N,d3; (R)-2-Amino-3-mercaptopropionic acid-15N,d3; Stable Isotope Labeled L-Cysteine; 15N,D3-L-Cys
EINECS	Contact for details

Quality Control

Our L-Cysteine-15N,D3 is manufactured under controlled conditions to ensure the highest isotopic and chemical purity. Each batch is subjected to rigorous analytical testing, including NMR, HPLC, and Mass Spectrometry, to confirm isotopic enrichment, chiral purity, and chemical identity. Certificates of Analysis (COA) detailing isotopic purity (atom % 15N and % D), chemical purity, and specific analytical data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This material is hygroscopic (moisture-sensitive); ensure the container is sealed under an inert atmosphere after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (15N)	≥ 98 atom % 15N
Isotopic Purity (D)	≥ 98 atom % D
Optical Rotation	[α]D20: +8.0° to +10.0° (c=1 in 1N HCl)
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.