Description

N6-(2-Hydroxyethyl-D4)-2'-Deoxyadenosine is a deuterated analog of 2'-deoxyadenosine, a fundamental nucleoside building block. This compound is specifically engineered for use as a stable isotope-labeled internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and reproducibility in analytical measurements. It is an essential reagent for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors, particularly for pharmacokinetic studies, metabolomics, and the development of novel therapeutics.

Application

• Quantitative LC-MS/MS Analysis: Serves as a critical internal standard for the precise quantification of 2'-deoxyadenosine and related metabolites in biological matrices like plasma, urine, and tissue homogenates.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Enables accurate tracking of drug metabolism and nucleotide analog therapeutics, such as antiviral or anticancer agents, within preclinical and clinical research.
• Biomarker Discovery & Validation: Used in metabolomic and proteomic workflows to identify and quantify disease-specific biomarkers with high confidence, correcting for matrix effects and instrument variability.
• Diagnostic Assay Development: Integral to the development and calibration of high-sensitivity clinical diagnostic tests that measure nucleotide levels, such as inborn errors of metabolism or cellular proliferation assays.
• Drug Impurity Profiling: Supports the identification and quantification of process-related impurities and degradants in Active Pharmaceutical Ingredient (API) manufacturing for regulatory compliance.
• Stable Isotope Labeling Studies: Facilitates metabolic flux analysis and tracing of nucleotide synthesis and salvage pathways in cell culture and animal models.

Basic Information

Product Name	N6-(2-Hydroxyethyl-D4)-2'-Deoxyadenosine
CAS No.	1644566-72-2
Molecular Formula	C₁₂H₁₃D₄N₅O₄
Molecular Weight	299.31 g/mol
Synonyms	2'-Deoxy-N6-(2-hydroxyethyl-d4)adenosine; N6-(2-Hydroxyethyl-1,1,2,2-d4)-2'-deoxyadenosine; dA(Hydroxyethyl-d4); Deuterated 2'-Deoxyadenosine Derivative; 2'-dA-N6-(2-Hydroxyethyl-d4); N6-(2-Hydroxyethyl-d4)-2'-dA; Stable Isotope-Labeled 2'-Deoxyadenosine; [D4]-N6-(2-Hydroxyethyl)-2'-deoxyadenosine
EINECS	Contact for details

Quality Control

Every batch of N6-(2-Hydroxyethyl-D4)-2'-Deoxyadenosine is manufactured and tested under strict quality management systems. We guarantee high chemical purity and isotopic enrichment, supported by comprehensive analytical data including NMR, HPLC, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, isotopic purity (D-enrichment), and absence of critical impurities. Our quality commitment ensures the reliability required for sensitive analytical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under an inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.