Description

Pfi-2 (Hydrochloride) is a potent and selective small-molecule inhibitor of the P-TEFb complex, specifically targeting the CDK9/Cyclin T1 interaction. This compound is a critical research tool for studying transcriptional regulation, cell cycle control, and apoptosis in various disease models. It is primarily utilized by pharmaceutical researchers, academic institutions, and biotechnology companies engaged in oncology, virology, and molecular biology research to investigate novel therapeutic pathways.

Application

• Biochemical Research: A key reagent for studying the mechanism of transcriptional elongation and the role of CDK9 in gene expression.
• Oncology Research: Used in vitro and in vivo to investigate cancer cell proliferation, survival, and to evaluate potential anti-cancer therapeutics targeting transcription.
• Virology Studies: Applied in research on viral replication, particularly for viruses like HIV that hijack host transcriptional machinery, to explore novel antiviral strategies.
• Signal Transduction Analysis: Serves as a specific probe to dissect the P-TEFb signaling pathway and its downstream effects in various cell types.
• Drug Discovery & Development: Used as a reference standard or lead compound in high-throughput screening and structure-activity relationship (SAR) studies for CDK9 inhibitors.
• Cellular Mechanism Studies: Employed to understand cellular responses to transcriptional stress, including the induction of apoptosis in specific cancer cell lines.

Basic Information

Product Name	Pfi-2 (Hydrochloride)
CAS No.	1627607-87-7
Molecular Formula	C₂₀H₂₅ClN₆O₂
Molecular Weight	416.90 g/mol
Synonyms	Pfi-2 HCl; N-(4-(4-(Cyclopentylamino)-7H-pyrrolo[2,3-d]pyrimidin-5-yl)phenyl)acetamide hydrochloride; CDK9 Inhibitor Pfi-2 hydrochloride; Pfi-2 hydrochloride salt; 5-(4-Acetamidophenyl)-N-cyclopentyl-7H-pyrrolo[2,3-d]pyrimidin-4-amine hydrochloride
EINECS	Contact for details

Quality Control

Our Pfi-2 (Hydrochloride) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure high chemical purity and structural identity. Certificates of Analysis (COA) detailing purity, potency, and full analytical data are provided with every shipment to guarantee reproducible research results and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.5%
Solubility	Soluble in DMSO, Methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.