Description

(S)-4'-Nitro-Blebbistatin is a potent and selective small-molecule inhibitor of non-muscle myosin II (NMMII), specifically engineered for enhanced research utility. This compound matters for its critical role in studying cellular processes such as cytokinesis, cell migration, and morphology by precisely blocking myosin II ATPase activity. Researchers in pharmaceutical R&D, academic biochemistry, and cell biology laboratories need it as a key pharmacological tool for investigating cytoskeletal dynamics and developing potential therapeutic strategies.

Application

• Biochemical Research: Used as a selective inhibitor in enzymatic assays to study the kinetics and function of non-muscle myosin II ATPase.
• Cell Biology Studies: Essential for investigating cytokinesis, cell division, and actin-myosin contractility in various cell lines.
• Pharmacological Tool Compound: Serves as a reference standard and active agent in high-throughput screening (HTS) for drug discovery programs targeting cytoskeletal disorders.
• Mechanobiology Research: Applied to explore the role of mechanical forces in cell signaling, adhesion, and migration.
• Neuroscience Research: Utilized to study the role of myosin II in neuronal growth cone guidance and synaptic function.
• Intermediate for Analog Synthesis: Acts as a key chiral building block for the synthesis of novel blebbistatin derivatives with modified pharmacological profiles.

Basic Information

Product Name	(S)-4'-Nitro-Blebbistatin
CAS No.	1621326-32-6
Molecular Formula	C18H16N4O5
Molecular Weight	368.35 g/mol
Synonyms	(S)-4'-Nitroblebbistatin; (S)-Nitroblebbistatin; (11S)-6'-Nitro-11,12-dihydro-6'H,11H-[1,3]dioxolo[4,5-g]pyrido[2,1-b]quinazolin-11-ol; (S)-4'-Nitro-Blebbistatin; 4'-Nitroblebbistatin (S-enantiomer); (-)-4'-Nitroblebbistatin; Myosin II Inhibitor (S)-4'-Nitro-Blebbistatin
EINECS	Contact for details

Quality Control

Our (S)-4'-Nitro-Blebbistatin is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) providing data for identity, purity, and impurity profiles. We adhere to rigorous internal standards and can support development toward cGMP-grade material upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. Allow the product to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (IR)	Spectrum matches reference
Purity (HPLC)	≥ 98.0%
Specific Rotation	Contact for details
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.