Description

Thz1 is a highly selective, covalent inhibitor of the transcriptional kinase TTK (also known as MPS1), widely used in oncology research for studying mitotic checkpoint mechanisms and synthetic lethality in cancer cell lines. Its significance lies in enabling reproducible, target-specific disruption of spindle assembly checkpoint signaling—critical for high-fidelity preclinical target validation and drug combination studies. Researchers in pharmaceutical R&D, academic cancer biology labs, and contract research organizations (CROs) rely on Thz1 CAS NO 1604810-83-4 for mechanistic studies, biomarker discovery, and resistance modeling.

Application

• Oncology drug discovery: Target validation and mechanism-of-action studies for TTK/MPS1 inhibitors
• Cell cycle research: Functional analysis of mitotic arrest, chromosome missegregation, and aneuploidy induction
• Combination therapy screening: Synergy assessment with antimitotics (e.g., paclitaxel), PARP inhibitors, or DNA-damaging agents
• CRISPR/Cas9 and RNAi validation: Orthogonal confirmation of TTK-dependent phenotypes
• Biomarker development: Identification of predictive signatures for TTK inhibitor sensitivity in solid tumors and hematologic malignancies
• Preclinical pharmacodynamic assay development: Phospho-histone H3 (Ser10) and MAD2 localization quantification
• High-content imaging workflows: Live-cell tracking of mitotic duration and error correction failure
• 3D organoid and patient-derived xenograft (PDX) model studies requiring precise, reversible target engagement

Basic Information

Product Name	Thz1
CAS No.	1604810-83-4
Molecular Formula	C₂₃H₁₉N₅O₂S
Molecular Weight	437.49 g/mol
Synonyms	THZ1; N-(4-(2-(4-Methylpiperazin-1-yl)ethoxy)-3-nitrophenyl)-5-(thiophen-2-yl)isoxazole-3-carboxamide; TTK inhibitor THZ1; MPS1 inhibitor THZ1; 5-(Thiophen-2-yl)-N-(4-(2-(4-methylpiperazin-1-yl)ethoxy)-3-nitrophenyl)isoxazole-3-carboxamide; THZ 1; THZ-1; TTK/MPS1 Inhibitor I; Compound THZ1
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch of Thz1 is verified for identity (HPLC retention time, IR, ¹H-NMR), purity (≥98.5% by HPLC), residual solvents (GC), heavy metals (ICP-MS), and water content (Karl Fischer). Testing aligns with ACS Reagent Grade and ISO 9001:2015 requirements for research-grade chemical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, maintain low ambient humidity (<30% RH) and avoid repeated exposure to air. Allow to equilibrate to room temperature in the sealed container before opening.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identity (IR)	Conforms to reference standard
Identity (HPLC)	Retention time matches reference standard (±0.2 min)
Assay (HPLC)	≥98.5%
Related Substances (HPLC)	Single impurity ≤0.3%; Total impurities ≤1.0%
Residual Solvents (GC)	Meets ICH Q3C Class 3 limits
Water Content (KF)	≤0.5%
Heavy Metals (ICP-MS)	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.