Description

Riboflavin-13C4,15N2 5-Phosphate is a stable isotope-labeled analog of Flavin Mononucleotide (FMN), a critical coenzyme in numerous biological redox reactions. This high-purity labeled compound is essential for advanced quantitative mass spectrometry and metabolic flux analysis, providing unparalleled accuracy in tracing biochemical pathways. It is primarily utilized by researchers and manufacturers in pharmaceutical development, clinical diagnostics, and nutritional science for precise, interference-free measurement of flavin-dependent processes.

Application

• Internal Standard for LC-MS/MS: Serves as a critical quantitative reference standard in liquid chromatography-tandem mass spectrometry for the accurate measurement of endogenous FMN and related metabolites in complex biological matrices.
• Metabolic Pathway Tracing (Fluxomics): Used in 13C and 15N metabolic flux analysis to elucidate the dynamics of flavin-dependent pathways in cell culture, microbial fermentation, and in vivo studies.
• Pharmaceutical R&D: Enables precise pharmacokinetic and pharmacodynamic studies of drugs that interact with flavoproteins or riboflavin metabolism.
• Clinical Research & Diagnostics: Facilitates the development of highly specific assays for diagnosing inborn errors of metabolism related to riboflavin responsiveness, such as multiple acyl-CoA dehydrogenase deficiency (MADD).
• Nutritional Science: Employed in advanced studies to quantify riboflavin bioavailability, tissue distribution, and turnover rates with high specificity.
• Enzyme Mechanism Studies: Provides a non-radioactive tool for probing the mechanism and kinetics of flavin-dependent oxidoreductases, dehydrogenases, and monooxygenases.

Basic Information

Item	Detail
Product Name	Riboflavin-13C4,15N2 5-Phosphate
CAS No.	1453471-52-7
Molecular Formula	13C415N2C13H18N4O9P
Molecular Weight	~469.26 (for labeled form)
Synonyms	FMN-13C4,15N2; Flavin Mononucleotide-13C4,15N2; Riboflavin 5'-Phosphate-13C4,15N2; Vitamin B2 Phosphate-13C4,15N2; 7,8-Dimethyl-10-ribitylisoalloxazine-5'-phosphate-13C4,15N2; Stable Isotope Labeled FMN; [13C4,15N2]-FMN
EINECS	Contact for details

Quality Control

Every batch of Riboflavin-13C4,15N2 5-Phosphate is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity through comprehensive analytical techniques including NMR, HPLC, and high-resolution mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting identity, purity, isotopic enrichment, and other critical parameters to ensure consistency and reliability for your research.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under desiccated conditions. This product is light-sensitive (store away from light). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to expected mass spectrum
Purity (HPLC)	≥ 95% (Chemical Purity)
Isotopic Enrichment	≥ 98 atom % 13C; ≥ 98 atom % 15N
Water Content (KF)	≤ 5.0 %
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.