Description

Mps1-In-5 is a potent and selective small-molecule inhibitor of the monopolar spindle 1 (Mps1/TTK) kinase, a key regulator of the spindle assembly checkpoint. This compound is a critical research tool for investigating cell cycle dynamics, chromosome segregation, and mitotic fidelity in oncology and cell biology studies. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and biotechnology companies focused on cancer therapeutics and fundamental cellular mechanisms.

Application

• Cancer Research & Drug Discovery: Used as a reference standard and pharmacological probe to study the role of Mps1 kinase in tumor cell proliferation and aneuploidy.
• Cell Biology Studies: Essential for exploring the spindle assembly checkpoint (SAC), mitotic exit, and chromosome instability in various cell lines.
• High-Throughput Screening (HTS): Serves as a positive control or tool compound in assay development and screening campaigns targeting kinase inhibitors.
• Mechanistic & Target Validation: Facilitates the validation of Mps1 as a therapeutic target in preclinical models of cancer.
• Biochemical Assays: Employed in in vitro kinase assays to determine IC50 values and selectivity profiles against other kinases.
• Apoptosis & Cell Cycle Analysis: Used to induce mitotic arrest and study subsequent cellular outcomes, including apoptosis and senescence.

Basic Information

Product Name	Mps1-In-5
CAS No.	1443763-60-7
Molecular Formula	C₂₀H₂₀N₆O₂
Molecular Weight	376.41 g/mol
Synonyms	Mps1-IN-5; TTK Inhibitor 5; MPS1/TTK Kinase Inhibitor; 2-[[4-[(4-Methylpiperazin-1-yl)methyl]phenyl]amino]-5-oxo-7H-pyrrolo[2,3-d]pyrimidine-6-carbonitrile; N-(4-((4-Methylpiperazin-1-yl)methyl)phenyl)-5-oxo-6-cyano-7H-pyrrolo[2,3-d]pyrimidin-2-amine
EINECS	Contact for details

Quality Control

Our Mps1-In-5 is manufactured under strict quality management systems. Every batch undergoes comprehensive analytical testing, including HPLC, NMR, and MS, to ensure high purity and structural identity. Certificates of Analysis (COA) with detailed chromatographic and spectroscopic data are provided to guarantee batch-to-batch consistency and support your research reproducibility.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C under desiccated conditions. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.