Description

Pitstop 2 is a highly specific, cell-permeable small molecule inhibitor of clathrin-mediated endocytosis. This compound is critical for researchers and developers in life sciences, providing a powerful tool to selectively disrupt and study intracellular trafficking pathways. Its primary value lies in enabling precise mechanistic studies in cell biology, virology, and neurobiology. Key industries that require this product include pharmaceutical R&D, academic research institutions, and biotechnology companies focused on drug delivery systems and viral entry mechanisms.

Application

• Mechanistic Cell Biology Research: Used to specifically inhibit clathrin-mediated endocytosis (CME) in live cells, allowing scientists to dissect the role of CME in various cellular processes.
• Virology and Antiviral Studies: Employed to block the clathrin-dependent entry of numerous viruses (e.g., influenza, dengue, SARS-CoV-2) into host cells, facilitating research into viral life cycles and potential therapeutic interventions.
• Neuroscience and Receptor Trafficking: Applied in studies of neurotransmitter receptor internalization (e.g., AMPA, GABA receptors) to understand synaptic plasticity, neuronal signaling, and related neurological disorders.
• Drug Delivery System Development: Serves as a control and tool compound for evaluating the cellular uptake mechanisms of novel nanocarriers, liposomes, and antibody-drug conjugates.
• Signal Transduction Pathway Analysis: Used to investigate how growth factor and hormone signaling (e.g., EGFR, transferrin) is regulated by receptor endocytosis and degradation.
• Cancer Research: Utilized to study the role of endocytosis in oncogenic signaling, tumor suppressor function, and the efficacy of chemotherapeutic agents.

Basic Information

Product Name	Pitstop 2
CAS No.	1419320-73-2
Molecular Formula	C₂₀H₂₀N₄O₃
Molecular Weight	364.40 g/mol
Synonyms	Pitstop 2; 5-[(2,3-Dihydro-1,4-benzodioxin-6-yl)carbonyl]-2-[(4-fluorophenyl)methyl]-1H-imidazo[4,5-c]pyridin-4-amine; 1H-Imidazo[4,5-c]pyridin-4-amine, 5-[(2,3-dihydro-1,4-benzodioxin-6-yl)carbonyl]-2-[(4-fluorophenyl)methyl]-; Clathrin Inhibitor Pitstop 2; CME Inhibitor Pitstop 2; Endocytosis Inhibitor Pitstop 2
EINECS	Contact for details

Quality Control

Every batch of Pitstop 2 is manufactured and analyzed under strict quality management protocols to ensure identity, purity, and consistency for research applications. Our standard material is characterized by multiple orthogonal analytical techniques, including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and batch-specific data is provided with each shipment to support your experimental reproducibility and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.