Description

Pitstop 2 is a potent and selective small molecule inhibitor of the AAA ATPase Vps4, a key enzyme in the endosomal sorting complexes required for transport (ESCRT) pathway. This compound is a critical research tool for investigating the molecular mechanisms of membrane remodeling, viral budding, and cytokinesis. It is primarily utilized by researchers in academic, pharmaceutical, and biotechnology laboratories focused on cell biology, virology, and oncology.

Application

• Biomedical Research: Fundamental studies on the ESCRT pathway's role in multivesicular body (MVB) formation, membrane scission, and cellular abscission.
• Virology Studies: Investigation of viral egress mechanisms, particularly for enveloped viruses (e.g., HIV-1, Ebola) that hijack the ESCRT machinery for budding from host cells.
• Cancer Research: Exploration of cell division and cytokinesis, as the ESCRT pathway is involved in the final separation of daughter cells.
• Neuroscience Research: Studies on neurodegenerative disease models where ESCRT dysfunction is implicated, such as in the processing of amyloid precursor protein.
• High-Content Screening: Used as a reference compound or positive control in cell-based assays screening for modulators of the ESCRT pathway or viral replication.
• Chemical Biology: A tool compound for validating targets and understanding the phenotypic consequences of specific Vps4 inhibition in various cellular contexts.

Basic Information

Product Name	Pitstop 2
CAS No.	1419093-54-1
Molecular Formula	C₂₀H₂₀N₄O₃
Molecular Weight	364.40 g/mol
Synonyms	Pitstop 2; 1-(4-(4-Methylpiperazin-1-yl)phenyl)-9H-pyrido[3,4-b]indol-3-one; Vps4 Inhibitor Pitstop 2; VPS4 ATPase Inhibitor; ESCRT Pathway Inhibitor Pitstop 2
EINECS	Contact for details

Quality Control

Our Pitstop 2 is manufactured under strict quality management protocols to ensure batch-to-batch consistency and reliability for research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity verification and NMR for structural confirmation. A Certificate of Analysis (COA) detailing identity, purity, and analytical data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.