Description

Pf-543 (Citrate) is a highly selective, cell-permeable inhibitor of sphingosine kinase 1 (SphK1), formulated as the citrate salt for enhanced aqueous solubility and stability in biological assay systems. It serves as a critical pharmacological tool for probing sphingolipid signaling pathways in preclinical research, enabling precise modulation of sphingosine-1-phosphate (S1P) production. This compound is essential for academic laboratories, biopharmaceutical R&D teams, and contract research organizations conducting mechanistic studies in oncology, inflammation, fibrosis, and immunomodulation. Pf-543 (Citrate) CAS NO 1415562-83-2 is supplied as a high-purity reference standard for reproducible experimental outcomes.

Application

• Target validation studies of SphK1 in cancer cell proliferation and migration assays
• In vitro investigation of S1P-mediated endothelial barrier function and vascular permeability
• Preclinical evaluation of sphingolipid pathway inhibition in murine models of rheumatoid arthritis and inflammatory bowel disease
• Co-treatment studies with chemotherapeutics to assess synergy in apoptosis induction
• High-content screening platforms for novel SphK1 modulators using Pf-543 (Citrate) as benchmark inhibitor
• Metabolomic profiling of sphingolipid intermediates in response to acute SphK1 blockade
• Crystallographic and biochemical characterization of SphK1–inhibitor binding interactions
• Quality control reference material for analytical method development (HPLC, LC-MS)

Basic Information

Product Name	Pf-543 (Citrate)
CAS No.	1415562-83-2
Molecular Formula	C27H32N4O9·C6H8O7
Molecular Weight	684.63 g/mol
Synonyms	PF-543 citrate; PF543 citrate salt; (2R,3S)-2-Amino-3-methoxy-4-(4-(pyridin-4-yl)thiazol-2-yl)butanoic acid citrate; Sphingosine kinase 1 inhibitor citrate; SK1 inhibitor citrate; N-(3-Hydroxy-1-adamantyl)-4-(4-methylpiperazin-1-yl)benzamide citrate (incorrectly associated — excluded); 2-Amino-3-methoxy-4-[4-(pyridin-4-yl)-1,3-thiazol-2-yl]butanoic acid citrate; PF-543 monohydrochloride citrate (misnomer — not applicable); PF-543 sodium citrate (not commercially used)
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch of Pf-543 (Citrate) is tested for identity (IR, HPLC retention time), assay (≥98.5% by HPLC), related substances (total impurities ≤1.5%), residual solvents (meets ICH Q3C guidelines), and heavy metals (≤10 ppm). Testing adheres to principles aligned with USP & EP monograph requirements for reference standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (nitrogen or argon) to prevent degradation. Due to its hygroscopic nature, maintain low-humidity conditions (<30% RH) and minimize exposure to ambient air during handling. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	98.5% – 101.0%
Related Substances	Total impurities ≤1.5%
Residual Solvents	Meets ICH Q3C Class 3 limits
Water Content (KF)	≤2.0%
Heavy Metals	≤10 ppm
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.