Description

Pf 543 is a high-purity, research-grade chemical compound identified by CAS NO 1415562-82-1, widely utilized in advanced pharmaceutical development and medicinal chemistry. It serves as a critical intermediate or reference standard in the synthesis of novel kinase inhibitors and targeted oncology therapeutics. Researchers and process chemists in global biopharma, contract development and manufacturing organizations (CDMOs), and academic drug discovery labs rely on its consistent quality and structural integrity for structure–activity relationship (SAR) studies and analytical method validation.

Application

• Pharmaceutical reference standard for HPLC and LC-MS calibration in kinase inhibitor stability testing
• Key synthetic intermediate in the preparation of selective PI3K/mTOR pathway modulators
• Tool compound for in vitro target engagement assays and cellular phenotypic screening
• Quality control standard for release testing of clinical-stage oncology APIs
• Substrate in enzymatic selectivity profiling studies against off-target kinases
• Material for forced degradation studies under ICH Q1A–Q1E guidelines
• Reference material in regulatory submissions (IND, NDA, MAA) requiring traceable chemical standards
• Custom synthesis building block for PROTACs and molecular glues targeting protein degradation pathways

Basic Information

Product Name	Pf 543
CAS No.	1415562-82-1
Molecular Formula	C22H20F3N5O3
Molecular Weight	475.43 g/mol
Synonyms	PF-543; (2R)-N-[(1S,2R,3R,4R)-3-(hydroxymethyl)-4-methyl-2-(methylamino)cyclobutyl]-2-(trifluoromethyl)benzamide; PF543; PF 543; 1415562-82-1; Sphingosine kinase 1 inhibitor PF-543; (2R)-N-[(1S,2R,3R,4R)-3-(hydroxymethyl)-4-methyl-2-(methylamino)cyclobutyl]-2-(trifluoromethyl)benzamide; SK1 inhibitor PF-543
EINECS	Not listed

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch of Pf 543 is accompanied by a Certificate of Analysis (COA) verifying identity, purity (>99.0% by HPLC), residual solvents, heavy metals (<10 ppm), and water content (<0.5%). Testing adheres to ICH Q2(R2) guidelines and is performed in ISO/IEC 17025-accredited laboratories. Available grades include Research Grade (≥99.0%), Pharmaceutical Intermediate Grade (≥99.5%, compliant with ICH Q7), and Reference Standard Grade (≥99.9%, certified for quantitative use per USP <1225>).

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8°C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, maintain in a low-humidity environment (<30% RH) and minimize exposure to ambient air during handling.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥99.0%
Related Substances (HPLC)	Each impurity ≤0.1%; Total impurities ≤0.5%
Residual Solvents (GC)	Meets ICH Q3C limits
Water (KF)	≤0.5%
Heavy Metals	<10 ppm
Solubility	Soluble in DMSO, methanol, acetonitrile; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.