Description

Pitstop 2 is a highly specific, cell-permeable small molecule inhibitor of clathrin-mediated endocytosis. This compound matters because it provides researchers with a powerful tool to selectively block the internalization of cell surface receptors and cargo, enabling critical studies in cell biology, signal transduction, and drug delivery mechanisms. It is essential for life science researchers, pharmaceutical developers, and academic institutions investigating endocytic pathways, receptor trafficking, and viral entry processes.

Application

• Cell Biology Research: Fundamental studies on clathrin-dependent endocytosis and intracellular trafficking.
• Signal Transduction Analysis: Investigating the role of receptor internalization in growth factor, cytokine, and GPCR signaling pathways.
• Drug Delivery & Uptake Studies: Researching mechanisms of nanoparticle, antibody, and therapeutic oligonucleotide entry into cells.
• Virology Research: Studying clathrin-mediated viral entry mechanisms for viruses like influenza and hepatitis C.
• Neuroscience: Exploring synaptic vesicle recycling and neurotransmitter receptor dynamics.
• Cancer Research: Investigating how endocytosis inhibition affects oncogenic signaling and receptor tyrosine kinase activity.
• Tool Compound: Used as a critical control and experimental agent in high-content screening and assay development.

Basic Information

Product Name	Pitstop 2
CAS No.	1332879-52-3
Molecular Formula	C₂₀H₂₀N₄O₃
Molecular Weight	364.40 g/mol
Synonyms	Pitstop 2; 5-[(4-Bromophenyl)methyl]-N-[(3,4-dihydro-2H-1-benzopyran-6-yl)methyl]-1,3,4-oxadiazol-2-amine; Clathrin Inhibitor Pitstop 2; Endocytosis Inhibitor Pitstop 2; 5-((4-Bromobenzyl))-N-((3,4-dihydro-2H-chromen-6-yl)methyl)-1,3,4-oxadiazol-2-amine; 1,3,4-Oxadiazol-2-amine, 5-[(4-bromophenyl)methyl]-N-[(3,4-dihydro-2H-1-benzopyran-6-yl)methyl]-
EINECS	Contact for details

Quality Control

Our Pitstop 2 is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and structural confirmation by NMR and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming identity, purity, and suitability for cell-based assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C under desiccant. This compound is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.