Description

α,4-Di-o-Acetyl Vanillylmandelic Acid-D3 Ethyl Ester CAS NO 1330265-93-4 is a high-purity, deuterium-labeled (D3) derivative of vanillylmandelic acid (VMA), a key catecholamine metabolite. This compound is specifically designed for use as an internal standard in quantitative mass spectrometry, providing superior accuracy and reliability in analytical workflows. It is an essential tool for clinical diagnostics, pharmaceutical research, and forensic toxicology laboratories requiring precise measurement of VMA and related metabolites.

Application

• Internal Standard for LC-MS/MS: Primary application as a stable isotope-labeled internal standard for the accurate quantification of vanillylmandelic acid (VMA) in biological matrices like urine and plasma.
• Clinical Diagnostics: Critical for the diagnosis and monitoring of neuroendocrine tumors, such as pheochromocytoma and neuroblastoma, by enabling precise VMA assays.
• Pharmaceutical R&D: Used in pharmacokinetic and metabolic studies of drugs affecting the catecholamine pathway.
• Forensic Toxicology: Supports the analysis of catecholamine metabolites in post-mortem investigations or substance abuse cases.
• Biomarker Research: Facilitates high-throughput screening and validation of VMA as a biomarker in various disease states.
• Method Development & Validation: Essential for developing, optimizing, and validating new liquid chromatography-mass spectrometry (LC-MS) methods to meet regulatory standards (CLIA, ISO 15189).

Basic Information

Item	Detail
Product Name	α,4-Di-o-Acetyl Vanillylmandelic Acid-D3 Ethyl Ester
CAS No.	1330265-93-4
Molecular Formula	C16H17D3O8
Molecular Weight	343.34 g/mol (approx.)
Synonyms	VMA-D3 Diacetyl Ethyl Ester; 3-Methoxy-4-hydroxymandelic Acid-D3 Diacetyl Ethyl Ester; 4-Hydroxy-3-methoxymandelic Acid-D3 Diacetyl Ethyl Ester; Deuterated VMA Internal Standard; D3-VMA Ethyl Ester Diacetate; Ethyl 2-(4-acetoxy-3-methoxyphenyl)-2-(acetoxy-d3)acetate; 1330265-93-4
EINECS	Contact for details

Quality Control

This high-value analytical standard is manufactured under strict quality control protocols to ensure isotopic purity and chemical integrity. Every batch is characterized using advanced techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided, detailing isotopic enrichment (D3, typically >98 atom % D), chemical purity (by HPLC), and identity confirmation. Our quality system supports compliance with ISO 9001 and GMP guidelines for laboratory reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer dedicated for chemicals. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (NMR/MS)	≥ 98 atom % D (D3)
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.