Description

Desethylene Ciprofloxacin-D4, Hydrochloride is a high-purity, isotopically labeled internal standard critical for advanced analytical chemistry. This deuterated compound is specifically engineered to provide unmatched accuracy and reliability in quantitative mass spectrometry, eliminating matrix effects and ensuring precise calibration. It is an essential reagent for pharmaceutical R&D laboratories, contract research organizations (CROs), and regulatory testing facilities engaged in method development, pharmacokinetic studies, and metabolite profiling of ciprofloxacin and related fluoroquinolone antibiotics.

Application

• Quantitative Bioanalysis: Serves as the primary internal standard in LC-MS/MS and GC-MS methods for the accurate quantification of ciprofloxacin in complex biological matrices such as plasma, serum, urine, and tissue homogenates.
• Pharmacokinetic & Metabolite Studies: Enables precise tracking of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research, facilitating reliable PK/PD modeling.
• Method Development & Validation: Used as a critical reference material to develop, optimize, and validate robust analytical methods in compliance with ICH, FDA, and EMA guidelines.
• Quality Control & Impurity Profiling: Employed in pharmaceutical quality control laboratories to assay active pharmaceutical ingredients (APIs) and identify or quantify related substances and degradation products.
• Environmental & Food Safety Testing: Applied in residue analysis to detect and quantify trace levels of fluoroquinolone antibiotics in environmental water samples, animal-derived food products, and agricultural commodities.
• Stability-Indicating Assays: Crucial for conducting forced degradation studies and establishing the stability profile of ciprofloxacin formulations under various stress conditions.

Basic Information

Product Name	Desethylene Ciprofloxacin-D4, Hydrochloride
CAS No.	1330261-13-6
Molecular Formula	C17H14D4F N3O3 • HCl
Molecular Weight	367.80 g/mol (for free base C17H14D4F N3O3)
Synonyms	1-Cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic Acid-d4, Hydrochloride; Ciprofloxacin-d4 Desethylene Hydrochloride; Ciprofloxacin-ethylenediamine-N-d4, Hydrochloride; 1-Cyclopropyl-6-fluoro-7-(piperazin-1-yl)-4-oxo-1,4-dihydroquinoline-3-carboxylic Acid-d4 Hydrochloride; Deuterated Ciprofloxacin Internal Standard; Ciprofloxacin-d4 (N-Deethylated) Hydrochloride; [2H4]-Ciprofloxacin Desethylene Hydrochloride
EINECS	Contact for details

Quality Control

Every batch of Desethylene Ciprofloxacin-D4, Hydrochloride is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure exceptional quality and isotopic purity. Our quality assurance protocols are designed to meet the stringent requirements of analytical reference standard applications. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing results for assay, isotopic enrichment, chemical purity, and identification. We support compliance with cGMP and ISO 9001 standards for traceable and reliable material supply.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive) and must be kept in a dry environment. For long-term storage, consider desiccant conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.