Description

Phenylbutazone-13C12 CAS NO 1325559-13-4 is a stable isotope-labeled analog of the non-steroidal anti-inflammatory drug (NSAID) phenylbutazone, where twelve carbon atoms are replaced with the carbon-13 isotope. This compound is essential as an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and reliability in analytical measurements. It is primarily required by pharmaceutical research laboratories, contract research organizations (CROs), and regulatory testing facilities for drug metabolism studies, pharmacokinetic analysis, and bioanalytical method development and validation.

Application

• Internal Standard for LC-MS/MS: Used as a critical reference compound in liquid chromatography-tandem mass spectrometry for the precise quantification of phenylbutazone and its metabolites in biological matrices.
• Drug Metabolism and Pharmacokinetics (DMPK): Facilitates accurate tracking and measurement of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical studies.
• Bioanalytical Method Development: Serves as a stable isotope-labeled internal standard to enhance method specificity, accuracy, and to compensate for sample preparation variability.
• Veterinary Pharmaceutical Analysis: Employed in residue testing and pharmacokinetic studies for phenylbutazone in food-producing animals to ensure food safety and regulatory compliance.
• Forensic and Anti-Doping Analysis: Provides a reliable isotopic pattern for the definitive identification and quantification of phenylbutazone in forensic toxicology and sports doping control.
• Pharmaceutical Quality Control: Supports impurity profiling and stability-indicating assay methods for phenylbutazone in finished drug products.

Basic Information

Product Name	Phenylbutazone-13C12
CAS No.	1325559-13-4
Molecular Formula	¹³C₁₂C₇H₁₈N₂O₂
Molecular Weight	~326.3 (based on ¹³C substitution)
Synonyms	4-Butyl-1,2-diphenyl-3,5-pyrazolidinedione-13C12; [13C12]-Phenylbutazone; 1,2-Diphenyl-4-butyl-3,5-pyrazolidinedione-13C12; Butazolidin-13C12; Isotopically Labeled Phenylbutazone; Phenylbutazone (13C12); 13C12-Labeled Phenylbutazone; EC 688
EINECS	Contact for details

Quality Control

Our Phenylbutazone-13C12 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for demanding analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment, chemical purity, and identity confirmation via advanced spectroscopic techniques. We adhere to relevant industry standards for reference materials to guarantee reliable and reproducible performance in your laboratory.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥98% (chemical purity)
Isotopic Enrichment	≥99 atom % 13C
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.