Description

Rac-N'-Nitrosonornicotine 1-n-Oxide is a nitrosamine derivative of nornicotine, a key alkaloid found in tobacco. This compound is of significant interest in analytical and toxicological research due to its role as a potential metabolite and biomarker for tobacco-specific nitrosamine (TSNA) exposure. It serves as a critical reference standard and analytical intermediate for laboratories focused on carcinogen studies, regulatory compliance testing, and substance characterization.

Application

• Analytical Reference Standard: Primary use as a certified standard for the quantification of tobacco-specific nitrosamines (TSNAs) in various matrices via HPLC, LC-MS, or GC-MS.
• Toxicology & Carcinogenicity Research: Used in in vitro and in vivo studies to investigate the metabolic pathways, biological activity, and health impacts of nitrosamine exposure.
• Biomarker Development: Critical for developing and validating analytical methods to detect and measure TSNA metabolites in biological samples (e.g., urine, blood) for exposure assessment.
• Pharmaceutical Impurity Profiling: Serves as a relevant impurity standard in the analysis of nicotine-containing pharmaceutical products to ensure compliance with stringent regulatory limits for genotoxic impurities.
• Environmental & Food Safety Testing: Applied in methods to screen for nitrosamine contamination in environmental samples, food products, and agricultural commodities.
• Chemical Synthesis Intermediate: Used as a building block or precursor in organic synthesis for creating novel compounds for research purposes.

Basic Information

Product Name	Rac-N'-Nitrosonornicotine 1-n-Oxide
CAS No.	1314916-69-2
Molecular Formula	C9H11N3O2
Molecular Weight	193.20 g/mol
Synonyms	N'-Nitrosonornicotine 1-N-Oxide (racemic); NNN N-Oxide (racemic); rac-NNN 1'-N-Oxide; 1-(3-((2S,5R)-5-Methyl-1-oxidopyrrolidin-2-yl)pyridin-4-yl)diazen-1-ium-1,2-diolate (racemic); Nornicotine, N'-nitroso-, 1-oxide (racemic); TSNA Reference Standard; Tobacco-specific nitrosamine oxide.
EINECS	Contact for details

Quality Control

Our high-purity reference materials are manufactured and handled under strict quality-controlled conditions. Each batch undergoes comprehensive analytical characterization using techniques including HPLC, NMR, and MS to confirm identity and purity. A detailed Certificate of Analysis (COA) is provided with each shipment, containing batch-specific data on purity, impurities, and chromatographic profiles. We support compliance with ICH Q3 guidelines for genotoxic impurities.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a cool, dry, and well-ventilated area. For long-term storage, consider storing under inert atmosphere at -20°C to maximize stability.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.