Description

(-N,o-Di-Desmethyl Tramadol-D3 is a deuterated analog of a key tramadol metabolite, specifically designed for use as an internal standard in analytical chemistry. This compound is critical for ensuring the accuracy and reliability of quantitative analyses in forensic, clinical, and pharmaceutical research. It is primarily utilized by analytical laboratories, contract research organizations (CROs), and pharmaceutical companies engaged in drug metabolism and pharmacokinetics (DMPK) studies, bioanalytical method development, and forensic toxicology.

Application

• Internal Standard for LC-MS/MS: Essential for the precise quantification of tramadol and its metabolites in biological matrices (e.g., plasma, urine, serum) using liquid chromatography-tandem mass spectrometry.
• Forensic Toxicology: Used in the analysis of post-mortem and human performance samples to confirm and quantify drug exposure with high specificity.
• Clinical Research: Supports pharmacokinetic and bioequivalence studies by providing a stable, isotopically labeled reference for accurate metabolite profiling.
• Pharmaceutical Development: Aids in the development and validation of bioanalytical methods for new drug formulations containing tramadol or related compounds.
• Reference Material: Serves as a certified reference material (CRM) for calibrating instruments and ensuring method compliance with regulatory guidelines (e.g., FDA, EMA).
• Doping Control: Employed in sports anti-doping laboratories to detect and confirm the presence of tramadol, a substance monitored in athletic competitions.

Basic Information

Product Name	(-N,o-Di-Desmethyl Tramadol-D3
CAS No.	1261398-22-4
Molecular Formula	C₁₅H₂₀D₃NO₂
Molecular Weight	249.36 g/mol
Synonyms	O-Desmethyl-N-Desmethyl Tramadol-D3; N,O-Didesmethyltramadol-D3; Didesmethyltramadol-D3; Tramadol Metabolite M5-D3; 2-[(Dimethylamino)methyl]-1-(3-methoxyphenyl)cyclohexanol-D3; UNII-9I0K6J2FDI (deuterated form); Tramadol-d3 metabolite; Internal Standard for Tramadol Analysis.
EINECS	Contact for details

Quality Control

Every batch of (-N,o-Di-Desmethyl Tramadol-D3 is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data for identity, purity (HPLC), isotopic enrichment (D-content), and specific tests for residual solvents and impurities. Our quality protocols are designed to meet the stringent requirements of GMP/GLP-compliant laboratories, ensuring reliability for regulatory submissions.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label or COA. Keep the container in a dry, cool, and well-ventilated place. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-content)	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.