Description

1-Hydroxymidazolam is a key pharmacologically active metabolite of the sedative-hypnotic agent midazolam. This compound is of significant importance in pharmaceutical research and development, particularly for pharmacokinetic studies, metabolic profiling, and the development of analytical reference standards. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and manufacturers of diagnostic kits and reference materials focused on drug metabolism and forensic toxicology.

Application

• Pharmaceutical Reference Standard: Serves as a certified reference material (CRM) for the quantification and qualification of midazolam and its metabolites in biological matrices.
• Metabolite Research: Critical for in vitro and in vivo studies investigating the metabolic pathways, pharmacokinetics, and pharmacodynamics of midazolam.
• Analytical Method Development: Used as a primary standard to develop and validate sensitive analytical methods, such as LC-MS/MS and HPLC, for clinical and forensic toxicology.
• Drug Interaction Studies: Employed in research to understand how other drugs affect the metabolism of midazolam, a known cytochrome P450 3A4 substrate.
• Impurity Profiling: Acts as a specified impurity standard in the quality control of midazolam active pharmaceutical ingredient (API) and finished dosage forms.
• Forensic Analysis: Essential for the accurate identification and confirmation of midazolam use in forensic and workplace drug testing programs.

Basic Information

Product Name	1-Hydroxymidazolam
CAS No.	1261396-36-4
Molecular Formula	C18H13ClFN3O
Molecular Weight	341.77 g/mol
Synonyms	α-Hydroxymidazolam; 1'-Hydroxymidazolam; 8-Chloro-6-(2-fluorophenyl)-1-methyl-4H-imidazo[1,5-a][1,4]benzodiazepin-4-ol; Midazolam Metabolite; 1-OH-Midazolam; U-44900; Midazolam 1-Hydroxy Metabolite
EINECS	Contact for details

Quality Control

Our 1-Hydroxymidazolam is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including identification by spectroscopic methods (IR, NMR, MS), purity determination by HPLC, and control of related substances. A detailed Certificate of Analysis (COA) is provided with each shipment, ensuring traceability and compliance with your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Residual Solvents (GC)	Complies with ICH Q3C guidelines
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.