Description

16α-Estriol-13C3 (16α-Hydroxyestradiol-[2,3,4-13C3]) (Certimass Solution) is a stable isotope-labeled analog of the endogenous estrogen metabolite 16α-hydroxyestradiol, specifically enriched with carbon-13 at three positions. This compound is a critical tool for quantitative mass spectrometry, enabling precise and accurate measurement of estriol and related hormone metabolites in complex biological matrices. It is essential for researchers and laboratories in clinical diagnostics, endocrinology research, and pharmaceutical development who require the highest standards of analytical accuracy for method validation and internal standardization.

Application

• Internal Standard for LC-MS/MS: Primary application as a quantitative internal standard for the analysis of estriol and its metabolites in serum, plasma, and urine.
• Clinical Research & Diagnostics: Used in developing and validating assays for hormone profiling, pregnancy monitoring, and endocrine disorder studies.
• Metabolomics & Biomarker Discovery: Facilitates accurate quantification in studies investigating estrogen metabolism pathways and their role in health and disease.
• Pharmaceutical R&D: Employed in pharmacokinetic and drug metabolism studies for compounds that interact with estrogen pathways.
• Reference Material: Serves as a certified reference material (CRM) for calibrating analytical instruments and ensuring data traceability.
• Forensic Toxicology: Applied in confirmatory testing for endogenous steroid levels in anti-doping and forensic analyses.

Basic Information

Product Name	16α-Estriol-13C3 (16α-Hydroxyestradiol-[2,3,4-13C3]) (Certimass Solution)
CAS No.	1255639-56-5
Molecular Formula	C18H24O3 (with three 13C atoms at positions 2, 3, 4)
Molecular Weight	~291.4 g/mol (exact mass depends on isotopic enrichment)
Synonyms	16α-Hydroxyestradiol-2,3,4-13C3; 16α-Estriol-13C3; Estra-1,3,5(10)-triene-3,16α,17β-triol-2,3,4-13C3; 16α-Hydroxy-17β-estradiol-13C3; Isotopically Labeled Estriol; Stable Isotope-Labeled Internal Standard; CRM for Estriol Analysis.
EINECS	Contact for details

Quality Control

Every batch of our Certimass Solution is produced and analyzed under strict quality protocols to ensure the highest isotopic and chemical purity required for sensitive mass spectrometry applications. Our quality commitment includes verification of isotopic enrichment (Atom % 13C), chemical purity, and solution concentration. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing batch-specific results from techniques such as NMR, HPLC, and MS. We support compliance with ISO 17034 and ISO/IEC 17025 requirements for reference material producers and testing laboratories.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store the sealed vial at 2-8°C. The solution is light-sensitive (store away from light). Allow the solution to equilibrate to room temperature before use and handle under standard laboratory conditions to prevent contamination.

Specification

Item	Specification
Appearance	Clear, colorless to pale yellow solution
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to expected mass
Isotopic Enrichment	≥ 99% 13C at each labeled position
Chemical Purity (HPLC)	≥ 98.0%
Concentration	Typically 0.1 mg/mL in solvent (e.g., methanol, acetonitrile)
Solvent	As specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.