Description

Hypoxanthine-13C2,15N is a stable isotope-labeled analog of the purine base hypoxanthine, specifically enriched with carbon-13 and nitrogen-15 at key positions. This compound is essential as a high-purity internal standard and tracer for precise quantitative analysis in mass spectrometry-based metabolomic and pharmacokinetic studies. It is primarily required by research institutions, pharmaceutical developers, and CROs (Contract Research Organizations) engaged in advanced drug metabolism, biomarker discovery, and nucleotide pathway research.

Application

• Mass Spectrometry Internal Standard: Used for accurate quantification of endogenous hypoxanthine in complex biological matrices like plasma, urine, and tissue homogenates.
• Drug Metabolism and Pharmacokinetics (DMPK): Serves as a critical tracer to study the metabolic fate and clearance pathways of purine-based pharmaceuticals.
• Metabolomics and Flux Analysis: Enables the tracing of purine salvage and synthesis pathways in cellular and in vivo models to understand metabolic disorders.
• Biomarker Research: Facilitates the development and validation of LC-MS/MS assays for hypoxanthine as a potential biomarker for conditions like ischemia, gout, or certain enzyme deficiencies.
• Nucleotide and Nucleic Acid Research: Employed in studies investigating purine biosynthesis, degradation, and the role of hypoxanthine in DNA/RNA metabolism.
• Quality Control in Diagnostic Kit Manufacturing: Acts as a reference material in calibrators and controls for clinical diagnostic assays measuring purine metabolites.

Basic Information

Product Name	Hypoxanthine-13C2,15N
CAS No.	1246820-04-1
Molecular Formula	C513C2H4N15N3O
Molecular Weight	139.09 g/mol (for labeled form)
Synonyms	1H-Purin-6-ol-13C2,15N; 6-Hydroxypurine-13C2,15N; Hypoxanthine (13C2, 15N); [13C2,15N]-Hypoxanthine; 6-Oxopurine-13C2,15N; Purine-6-ol-13C2,15N; Stable Isotope Labeled Hypoxanthine; Hypoxanthine-1,3-13C2,7-15N
EINECS	Contact for details

Quality Control

Every batch of Hypoxanthine-13C2,15N is manufactured and analyzed under strict quality systems. We ensure high chemical and isotopic purity through advanced analytical techniques including NMR, HPLC, and mass spectrometry. Certificates of Analysis (COA) are provided with each shipment, detailing batch-specific results for identity, purity, isotopic enrichment, and solvent content. Our production adheres to cGMP principles suitable for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, consider keeping the product at -20°C under desiccant to maintain stability and isotopic integrity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C; ≥ 99 atom % 15N
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.