Description

Carboxyphosphamide-D4 CAS NO 1246817-74-2 is a deuterium-labeled analog of carboxyphosphamide, a key metabolite of the chemotherapeutic agent cyclophosphamide. This stable isotope-labeled compound is essential for advanced analytical applications, providing an internal standard for precise quantification and metabolic pathway elucidation. It is primarily utilized by pharmaceutical researchers, contract research organizations (CROs), and diagnostic developers engaged in drug metabolism and pharmacokinetics (DMPK) studies, bioanalytical method development, and clinical research.

Application

• Internal Standard for LC-MS/MS: Serves as a critical deuterated internal standard in liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the accurate quantification of cyclophosphamide and its metabolites in biological matrices.
• Drug Metabolism Studies (DMPK): Used in preclinical and clinical research to trace metabolic pathways, study biotransformation, and assess the pharmacokinetic profile of cyclophosphamide.
• Bioanalytical Method Development and Validation: Essential for developing and validating robust, sensitive, and specific analytical methods in compliance with FDA and ICH guidelines.
• Clinical Research and Therapeutic Drug Monitoring (TDM): Supports studies monitoring drug and metabolite levels in patients to optimize dosing regimens and minimize toxicity.
• Pharmacology and Toxicology Research: Employed in investigative studies to understand the mechanisms of action, efficacy, and safety profile of cyclophosphamide-based therapies.
• Reference Material for Quality Control: Acts as a high-purity certified reference material (CRM) for quality assurance in analytical laboratories.

Basic Information

Product Name	Carboxyphosphamide-D4
CAS No.	1246817-74-2
Molecular Formula	C7H11D4Cl2N2O4P
Molecular Weight	289.10 g/mol (for D4 isotopologue)
Synonyms	4-[Bis(2-chloroethyl)amino]-2-deuterio-2-(trideuteriomethyl)-4-oxo-1,3,2λ5-oxazaphosphinane-2-carboxylic acid; Carboxy Cyclophosphamide-D4; 2-Deuterio-2-(trideuteriomethyl)-4-[bis(2-chloroethyl)amino]-4-oxo-1,3,2λ5-oxazaphosphinane-2-carboxylic acid; Cyclophosphamide Carboxylic Acid Metabolite-D4; Deutero Carboxyphosphamide; Stable Isotope Labeled Carboxyphosphamide
EINECS	Contact for details

Quality Control

Our Carboxyphosphamide-D4 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for critical research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing results for isotopic purity (D-enrichment), chemical purity (by HPLC), and identification (by NMR and MS). We adhere to relevant industry standards for reference materials, and custom synthesis to meet specific pharmacopeial or client specifications is available.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated freezer. For long-term storage under inert atmosphere, the product is hygroscopic and sensitive to temperature fluctuations. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Chemical Purity (by NMR)	≥ 97.0%
Residual Solvents (GC)	Complies with ICH limits
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.