Description

5-Aminoimidazole-4-Carboxamide-13C2,15N Hydrochloride Salt is a stable, isotopically labeled derivative of the key metabolic intermediate AICA ribotide. This compound is of critical importance for use as an internal standard in quantitative mass spectrometry, ensuring the highest accuracy and reproducibility in analytical workflows. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in metabolomics, pharmacokinetic studies, and stable isotope tracer research.

Application

• Quantitative LC-MS/MS Analysis: Serves as a high-purity internal standard for the accurate quantification of AICA ribotide and related purine biosynthesis intermediates in biological matrices.
• Metabolomics & Biomarker Discovery: Enables precise tracking of purine metabolic flux in cell cultures, tissues, and biofluids for disease mechanism studies.
• Drug Metabolism & Pharmacokinetics (DMPK): Used in stable isotope dilution assays to study the metabolism and clearance of purine-based pharmaceuticals.
• Enzyme Activity Assays: Acts as a labeled substrate or product analog in studies of enzymes like AICA ribotide transformylase/IMP cyclohydrolase (ATIC).
• NMR Spectroscopy: The 13C and 15N labels provide distinct signals for structural elucidation and dynamic studies of metabolic pathways.
• GMP/GLP-Compliant Manufacturing: Supports the development and validation of regulated bioanalytical methods for clinical trial sample analysis.

Basic Information

Product Name	5-Aminoimidazole-4-Carboxamide-13C2,15N Hydrochloride Salt
CAS No.	1246816-45-4
Molecular Formula	C413C2H8N315NO • HCl
Molecular Weight	Contact for details
Synonyms	AICA-13C2,15N Hydrochloride; 5-Amino-1H-imidazole-4-carboxamide-13C2,15N hydrochloride; AICAR-13C2,15N HCl; 5-Aminoimidazole-4-carboxamide-1,2-13C2,3-15N hydrochloride; Isotopically labeled AICA ribotide precursor; ZMP analog-13C2,15N; Acadesine-13C2,15N hydrochloride
EINECS	Contact for details

Quality Control

Every batch of our isotopically labeled materials is produced and tested under a strict quality management system. We guarantee high chemical and isotopic purity through advanced analytical techniques including NMR, HPLC, and high-resolution mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing assay, isotopic enrichment, and impurity profiles to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Enrichment	≥ 99 atom % 13C; ≥ 99 atom % 15N
Water Content (KF)	≤ 5.0 %
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.