Description

Rac Biotin-D4 CAS NO 1217850-77-5 is a high-purity, deuterium-labeled analog of biotin, a vital water-soluble B-complex vitamin. This stable isotope-labeled compound is essential for advanced analytical and research applications where precise quantification and metabolic pathway tracing are required. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and manufacturers of diagnostic kits for use as an internal standard in mass spectrometry (LC-MS/MS, GC-MS) and other sophisticated bioanalytical techniques.

Application

• Internal Standard for Quantitative Bioanalysis: Serves as a critical reference standard in LC-MS/MS and GC-MS methods for the accurate quantification of biotin and its metabolites in biological matrices (plasma, serum, tissue).
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Used to trace and elucidate the metabolic pathways, absorption, distribution, and excretion profiles of biotin and biotin-conjugated drugs.
• Clinical Diagnostics and Assay Development: Employed in the calibration and validation of high-sensitivity diagnostic assays, including vitamin deficiency tests and hormone immunoassays that utilize biotin-streptavidin technology.
• Nutritional and Dietary Supplement Research: Facilitates stable isotope dilution analysis for precise measurement of biotin content and bioavailability in fortified foods and supplements.
• Isotope-Labeled Reference Material: Provides a deuterated standard for quality control and method development in analytical laboratories to ensure data accuracy and regulatory compliance.

Basic Information

Product Name	Rac Biotin-D4
CAS No.	1217850-77-5
Molecular Formula	C10H12D4N2O3S
Molecular Weight	248.33 g/mol (for D4 isotopologue)
Synonyms	d4-Biotin (racemic); (+/-)-Biotin-d4; Vitamin B7-d4; Vitamin H-d4; Coenzyme R-d4; Hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanoic acid-d4; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-D4-pentanoic acid (racemic)
EINECS	Contact for details

Quality Control

Our Rac Biotin-D4 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for chemical and isotopic purity, NMR and MS for structural confirmation, and residual solvent analysis. We provide a comprehensive Certificate of Analysis (COA) detailing batch-specific results for identity, assay, isotopic enrichment, and impurities. Our quality commitment ensures the material meets the stringent requirements for use as a certified reference standard in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage under inert atmosphere, consider sealing under argon or nitrogen. Keep the container tightly sealed in a desiccator to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC-MS)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D4)	≥ 98.0 atom % D
Chemical Purity (by HPLC)	≥ 95.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.