Description

(S)-(-)-Blebbistatin o-Benzoate is a potent and selective cell-permeable inhibitor of non-muscle myosin II ATPase activity. This high-purity biochemical tool is critical for researchers investigating cytoskeletal dynamics, cell division, and mechanobiology. It is essential for pharmaceutical R&D, academic research, and biotechnology applications focused on developing novel therapeutics for conditions involving myosin II dysregulation.

Application

• Biochemical Research: A key tool for studying the role of non-muscle myosin II in cell motility, cytokinesis, and cellular contractility.
• Pharmaceutical Screening: Used in high-throughput assays to identify and validate new drug candidates targeting myosin II pathways.
• Mechanobiology Studies: Essential for investigating how mechanical forces are transduced into biochemical signals within cells and tissues.
• Cell Culture & Differentiation: Employed to manipulate cell morphology and adhesion in stem cell research and tissue engineering.
• Cancer Research: Used to probe the involvement of myosin II in tumor cell invasion, metastasis, and drug resistance.
• Neuroscience: Applied in studies of neuronal growth cone guidance and synaptic plasticity.

Basic Information

Item	Detail
Product Name	(S)-(-)-Blebbistatin o-Benzoate
CAS No.	1217832-61-5
Molecular Formula	C₂₇H₂₅N₃O₄
Molecular Weight	455.51 g/mol
Synonyms	(-)-Blebbistatin o-Benzoate; (S)-Blebbistatin Benzoate; Blebbistatin Benzoate Derivative; (S)-(-)-4-[(7-Nitro-4-benzofurazanyl)amino]-2,3-dihydro-1H-inden-1-yl benzoate; Myosin II Inhibitor (S)-(-)-Blebbistatin o-Benzoate; NMIIA Inhibitor; Bleb o-Benzoate
EINECS	Contact for details

Quality Control

Our (S)-(-)-Blebbistatin o-Benzoate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles. We adhere to rigorous analytical standards, utilizing HPLC, NMR, and MS for characterization, ensuring the product meets the exacting requirements of life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Meets ICH guidelines
Biological Activity (IC₅₀)	≤5 µM (Non-muscle Myosin IIA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.