Description

Rac trans-3'-Hydroxy Cotinine-3-Carboxylic Acid Methyl Ester is a high-purity chemical reference standard and synthetic intermediate of significant importance in analytical and pharmaceutical research. This compound is critical for the accurate quantification and metabolic study of nicotine and its derivatives, ensuring reliable data in clinical and forensic toxicology. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in drug development and substance abuse testing.

Application

• Pharmaceutical Reference Standard: Serves as a certified primary standard for calibrating analytical instruments in pharmacokinetic and bioequivalence studies.
• Metabolite Analysis: Used as a key analytical standard in LC-MS/MS and HPLC methods for the precise detection and quantification of nicotine metabolites in biological samples (e.g., urine, plasma).
• Diagnostic Reagent Manufacturing: An essential component in the production of immunoassays and test kits for nicotine exposure and smoking cessation monitoring.
• Organic Synthesis Intermediate: Employed in the research-scale synthesis of more complex nicotine analogs and labeled compounds for tracer studies.
• Forensic Toxicology: Provides a reliable benchmark for confirmatory testing in workplace drug testing and forensic investigations.
• Academic & Contract Research: Supports basic research into nicotine metabolism, addiction mechanisms, and the development of novel therapeutic agents.

Basic Information

Product Name	Rac trans-3'-Hydroxy Cotinine-3-Carboxylic Acid Methyl Ester
CAS No.	1217641-50-3
Molecular Formula	C12H14N2O4
Molecular Weight	250.25 g/mol
Synonyms	trans-3'-Hydroxycotinine methyl ester; 3'-Hydroxycotinine-3-carboxylic acid methyl ester; Methyl 3'-hydroxycotinine-3-carboxylate; (Rac)-trans-3'-Hydroxycotinine methyl ester; Nicotine metabolite derivative; 1-Methyl-5-(3-pyridyl)-2-pyrrolidinone-3-carboxylic acid, methyl ester, 3'-hydroxy-; Cotinine-3-carboxylic acid methyl ester, 3'-hydroxy-
EINECS	Contact for details

Quality Control

Every batch of Rac trans-3'-Hydroxy Cotinine-3-Carboxylic Acid Methyl Ester is manufactured and tested under strict quality management systems. We employ advanced chromatographic techniques to ensure identity, purity, and consistency, meeting the stringent requirements for reference standard materials. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profile is provided with each shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept in a desiccated environment to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.