Description

L-Histidine-15N3 is a stable isotope-labeled analog of the essential amino acid L-Histidine, where three nitrogen atoms (14N) are replaced with the 15N isotope. This compound is a critical tool for advanced quantitative proteomics, metabolic flux analysis, and pharmacokinetic studies, providing unparalleled accuracy as an internal standard in mass spectrometry. It is indispensable for researchers and manufacturers in pharmaceutical development, clinical diagnostics, and nutritional science who require precise, interference-free measurement of histidine metabolism and protein dynamics.

Application

• Quantitative Mass Spectrometry (MS) & Proteomics: Serves as an essential internal standard for the absolute quantification of proteins and peptides, eliminating matrix effects and ensuring data accuracy.
• Metabolic Pathway Tracing (Fluxomics): Used to track and model histidine incorporation and turnover in cellular systems, providing insights into metabolic disorders and disease mechanisms.
• Pharmaceutical R&D & Pharmacokinetics (PK): Enables precise measurement of drug metabolism and bioavailability in preclinical and clinical studies involving histidine-containing compounds.
• Clinical Diagnostics & Biomarker Discovery: Facilitates the development of highly accurate LC-MS/MS assays for diagnosing inborn errors of metabolism related to histidine, such as histidinemia.
• Nutritional Research: Employed in studies to assess protein digestibility, amino acid requirements, and the metabolic fate of histidine in dietary interventions.
• Stable Isotope Labeling by Amino acids in Cell culture (SILAC): A key reagent for heavy isotope labeling in cell culture to study protein synthesis, degradation, and post-translational modifications.

Basic Information

Product Name	L-Histidine-15N3
CAS No.	1217456-12-6
Molecular Formula	C6H915N3O2
Molecular Weight	158.13 g/mol (for 15N3)
Synonyms	(2S)-2-Amino-3-(1H-imidazol-4-yl)propanoic-15N3 acid; Histidine-15N3; L-Histidine (15N3); (S)-2-Amino-3-(4-imidazolyl)propionic-15N3 acid; H-His(15N3)-OH; FEMA 3694; L-(-)-Histidine-15N3
EINECS	Contact for details

Quality Control

Our L-Histidine-15N3 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for critical research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically ≥ 98 atom % 15N), chemical purity (by HPLC), and confirmation of stereochemical integrity. We adhere to relevant guidelines for the production of stable isotope-labeled compounds for analytical and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % 15N
Optical Rotation	[α]D20: +39.0° to +41.5° (c=1 in H2O)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.