Description

5-Methylnicotine-D3 is a deuterium-labeled isotopologue of 5-methylnicotine, a key structural analog of nicotine. This compound is of significant value as a stable isotope-labeled internal standard for precise quantitative analysis in mass spectrometry. It is primarily required by analytical laboratories and research institutions in the pharmaceutical, forensic, and toxicology sectors for method development, validation, and high-accuracy measurement of nicotine and its metabolites.

Application

• Internal Standard for LC-MS/MS: Essential for the accurate quantification of nicotine and its derivatives in complex biological matrices such as plasma, urine, and tissue homogenates.
• Metabolic Pathway Studies: Used as a tracer in pharmacokinetic and metabolic research to investigate the absorption, distribution, metabolism, and excretion (ADME) of nicotine analogs.
• Forensic Toxicology: Critical for developing and validating sensitive analytical methods to detect and quantify nicotine-related compounds in forensic casework.
• Pharmaceutical R&D: Supports the development of smoking cessation therapies and novel nicotinic receptor-targeting drugs by enabling precise bioanalytical measurements.
• Reference Material: Serves as a high-purity certified reference material (CRM) for calibrating analytical instruments and ensuring data integrity.
• Environmental Analysis: Potential use in tracking nicotine derivatives in environmental samples for pollution monitoring studies.

Basic Information

Item	Detail
Product Name	5-Methylnicotine-D3
CAS No.	1190016-33-1
Molecular Formula	C₁₁H₁₁D₃N₂
Molecular Weight	177.25 g/mol (approx.)
Synonyms	5-Methylnicotine-d3; 3-[(2S)-1-Methyl-2-pyrrolidinyl]pyridine-5-methyl-d3; Deuteronated 5-Methylnicotine; Nicotine-5-methyl-d3; (S)-5-Methylnicotine-d3; Isotopically Labeled 5-Methylnicotine; 5-(Trideuteriomethyl)nicotine; Internal Standard for Nicotine Analysis
EINECS	Contact for details

Quality Control

Every batch of 5-Methylnicotine-D3 is manufactured and analyzed under strict quality management protocols to ensure the highest standards of isotopic purity and chemical identity. Our products undergo rigorous quality testing, including advanced spectroscopic and chromatographic methods, to ensure compliance with research and analytical standards. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment, chemical purity, and analytical results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept under inert atmosphere or in a desiccated environment after opening to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-content)	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Residual Solvents (GC)	Complies with ICH guidelines
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.