Description

2'-Deoxy Cytidine-5,6-D2 is a deuterium-labeled isotopologue of the essential nucleoside 2'-deoxycytidine, where two hydrogen atoms at the 5 and 6 positions are replaced with deuterium. This stable isotope labeling is critical for advanced analytical and research applications, providing a non-radioactive tracer with distinct mass spectral properties. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, metabolic studies, and analytical chemistry for use as an internal standard and probe molecule.

Application

• Internal Standard for LC-MS/MS: Used as a high-purity, stable isotope-labeled internal standard in quantitative bioanalytical methods for pharmacokinetic and metabolomic studies.
• Pharmaceutical Research & Development: Serves as a key building block and tracer in the development and metabolic pathway analysis of antiviral and anticancer nucleoside analog drugs.
• Metabolic Flux Analysis: Enables precise tracking of nucleotide metabolism and DNA synthesis pathways in cellular and in vivo research models.
• Biochemical Research: Employed in mechanistic studies of enzyme kinetics, particularly for DNA polymerases and kinases involved in nucleoside metabolism.
• Diagnostic Kit Manufacturing: Incorporated as a calibrant or reference material in clinical diagnostic assays for genetic or metabolic disorders.
• Nuclear Magnetic Resonance (NMR) Spectroscopy: Acts as a useful probe in NMR studies due to the distinct properties of deuterium, aiding in structural elucidation.

Basic Information

Product Name	2'-Deoxy Cytidine-5,6-D2
CAS No.	1186526-91-9
Molecular Formula	C9H11D2N3O4
Molecular Weight	229.22 g/mol (for monoisotopic mass)
Synonyms	2'-Deoxy-5,6-dideuteriocytidine; Cytidine-5,6-d2, 2'-deoxy-; 1-(2-Deoxy-β-D-erythro-pentofuranosyl)-5,6-dideuterio-4-aminopyrimidin-2(1H)-one; dC-5,6-D2; 2'-Deoxycytidine-5,6-d2; Deoxycytidine-5,6-D2; 4-Amino-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5,6-dideuteriopyrimidin-2-one
EINECS	Contact for details

Quality Control

Every batch of 2'-Deoxy Cytidine-5,6-D2 is manufactured and tested under strict quality management systems. We ensure high chemical purity and isotopic enrichment through advanced analytical techniques including NMR, HPLC, and Mass Spectrometry. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for identity, purity (HPLC), isotopic purity (D-enrichment), and related substances. Our quality commitment supports research requiring reliable and consistent labeled compounds.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.