Description

Imidazole-13C,15N2 (Major) is a stable isotope-labeled analog of the fundamental heterocyclic compound imidazole, where the majority of molecules contain two 15N atoms and one 13C atom at the major labeling positions. This high-purity labeled standard is critical for ensuring accuracy and reliability in quantitative mass spectrometry and NMR spectroscopy analyses. It is an essential material for researchers and quality control laboratories in the pharmaceutical, agrochemical, and life science sectors who require definitive internal standards for method development, validation, and precise metabolite tracking.

Application

• Quantitative Mass Spectrometry (MS): Serves as a definitive internal standard for the accurate quantification of unlabeled imidazole and its derivatives in complex biological and chemical matrices.
• Metabolomics & Pharmacokinetic Studies: Enables precise tracking and quantification of drug metabolites and endogenous compounds in in vitro and in vivo studies.
• Nuclear Magnetic Resonance (NMR) Spectroscopy: Used as a probe in mechanistic and structural studies to elucidate reaction pathways and molecular interactions without signal interference.
• Pharmaceutical Impurity Profiling & Method Validation: Critical for developing and validating analytical methods to identify and quantify impurities and degradants in Active Pharmaceutical Ingredients (APIs).
• Environmental & Food Safety Analysis: Applied in the detection and quantification of imidazole-related compounds in environmental samples and food products.
• Isotope Dilution Analysis (IDA): The gold-standard technique for achieving high-accuracy results in analytical chemistry, compensating for sample loss and matrix effects.

Basic Information

Product Name	Imidazole-13C,15N2 (Major)
CAS No.	1173018-62-6
Molecular Formula	C313CH415N2
Molecular Weight	71.05 g/mol (for 13C315N2H4)
Synonyms	1,3-Diazole-13C,15N2; 1H-Imidazole-13C,15N2; Imidazole-1,3-15N2-2-13C; Imidazole-13C, 15N2 (major isotopologue); Imidazole (13C,15N2); Stable Isotope Labeled Imidazole; Isotopic Internal Standard Imidazole
EINECS	Contact for details

Quality Control

Every batch of Imidazole-13C,15N2 (Major) is manufactured and analyzed under strict quality systems. We provide comprehensive analytical data to ensure the product meets the stringent requirements for isotopic labeling purity and chemical purity. A detailed Certificate of Analysis (COA) is supplied with each shipment, reporting key parameters including isotopic enrichment (Atom % 13C and 15N), chemical purity by HPLC, and identity confirmation by NMR and MS. Our quality commitment supports compliance with cGMP and ISO 9001 standards for research and regulatory applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0% (Chemical Purity)
Isotopic Enrichment (13C)	≥ 99 atom % 13C
Isotopic Enrichment (15N)	≥ 98 atom % 15N
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.