Description

Z-Aevd-Fmk is a cell-permeable, irreversible caspase inhibitor widely used in apoptosis research and mechanistic studies of programmed cell death. It functions by covalently modifying the active-site cysteine residue of caspases, enabling precise temporal control over caspase activity in live-cell assays and preclinical models. Researchers in academic laboratories, biopharmaceutical R&D, and contract research organizations rely on Z-Aevd-Fmk CAS NO 1135688-47-9 for high-fidelity inhibition of caspase-3, -7, and related effector caspases in human and murine systems.

Application

• Apoptosis pathway validation in primary cell cultures and immortalized cell lines
• In vitro caspase activity profiling and inhibitor screening assays
• Neuroprotection studies targeting caspase-mediated neuronal death
• Ischemia-reperfusion injury models (e.g., cardiac, cerebral)
• Development of combination therapies with chemotherapeutic agents
• Quality control and functional testing of recombinant caspase enzymes
• Stabilization of apoptotic intermediates for proteomic analysis
• Preclinical pharmacodynamic biomarker development

Basic Information

Product Name	Z-Aevd-Fmk
CAS No.	1135688-47-9
Molecular Formula	C22H30F3N5O7
Molecular Weight	533.50 g/mol
Synonyms	Z-AEVD-FMK; Benzyloxycarbonyl-Ala-Glu-Val-Asp-fluoromethylketone; Z-AEVD(OMe)-FMK; Z-Ala-Glu-Val-Asp-FMK; Cbz-Ala-Glu-Val-Asp-FMK; Z-AEVD-FMK (human); Caspase-3 Inhibitor III; Z-AEVD-FMK (caspase-3/7 selective)
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for identity (IR, HPLC retention time), purity (≥98.0% by HPLC), and absence of microbial contamination. Testing follows ACS Reagent Grade and EP/USP general chapters where applicable, supporting use in regulated research environments.

Storage

Preserve in a tightly closed container, protected from light. Store at –20°C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, minimize exposure to ambient humidity; allow vial to equilibrate to room temperature in a desiccated environment before opening. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference standard
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.0%
Related Substances (HPLC)	Individual impurity ≤0.5%; Total impurities ≤2.0%
Residual Solvents (GC)	Meets ICH Q3C guidelines
Water (KF)	≤5.0%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.