Description

Robotnikinin is a potent and selective small-molecule inhibitor of the Hedgehog signaling pathway, a critical pathway in developmental biology and oncology. Its ability to specifically target the Smoothened receptor makes it a valuable research tool for studying cell differentiation, tissue patterning, and cancer stem cell biology. This high-purity compound is essential for pharmaceutical R&D, academic research in developmental biology, and the discovery of novel therapeutics targeting Hedgehog pathway-dependent cancers.

Application

• Biomedical Research: Fundamental studies on the Hedgehog (Hh) signaling pathway, its role in embryogenesis, and tissue homeostasis.
• Oncology Research: Investigation of Hh pathway dysregulation in cancers such as basal cell carcinoma, medulloblastoma, and pancreatic cancer.
• Drug Discovery & Screening: Used as a reference standard or active pharmacological agent in high-throughput screening (HTS) assays for novel Hh pathway inhibitors.
• Stem Cell Research: Probing the role of Hh signaling in the maintenance, proliferation, and differentiation of stem cells and cancer stem cells.
• Chemical Biology: A key tool compound for understanding protein-protein interactions and downstream effects within the Hh pathway.
• Academic & Institutional Labs: Essential reagent for universities and research institutes focused on developmental biology, molecular biology, and cancer research.

Basic Information

Product Name	Robotnikinin
CAS No.	1132653-79-2
Molecular Formula	C₂₀H₂₀N₂O₄
Molecular Weight	352.39 g/mol
Synonyms	N-[4-(2-Methyl-1H-indol-3-yl)-2-pyrimidinyl]-2,4-dichlorobenzamide; Robotnikinin (SMO antagonist); Hedgehog pathway inhibitor Robotnikinin; SMO inhibitor Robotnikinin; 2,4-Dichloro-N-[4-(2-methyl-1H-indol-3-yl)-2-pyrimidinyl]benzamide
EINECS	Contact for details

Quality Control

Our Robotnikinin is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation by NMR and MS. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.