Description

6-[2-Biotinylamidoethyl]-Dithiopropionamido]-4,8-Diaza-5,7-Diketoundecanoic Acid, Bis-n-Sulfosuccinimidyl Ester Disodium Salt is a highly specialized, water-soluble, heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is critical for creating stable, site-specific linkages between biomolecules, such as proteins and nucleic acids, and various surfaces or detection systems. It is an essential tool for researchers and developers in pharmaceutical discovery, in vitro diagnostics (IVD), and proteomics, where precise and reliable conjugation is paramount for assay performance and data integrity.

Application

• Antibody-Drug Conjugate (ADC) Development: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via cleavable disulfide linkages.
• Diagnostic Assay Manufacturing: Immobilization of capture antibodies or antigens onto microplates, beads, or biosensor surfaces for ELISA, lateral flow, and other immunoassays.
• Protein Labeling & Tagging: Introduction of biotin (for streptavidin capture) or other functional groups onto proteins for purification, detection, or imaging applications.
• Biomaterial & Surface Functionalization: Creating bioactive coatings on polymers, metals, or nanoparticles for tissue engineering, drug delivery, and biosensor platforms.
• Proteomics Research: Crosslinking proteins for interaction studies, structural analysis, or the preparation of stable protein complexes.
• Oligonucleotide Conjugation: Attaching peptides, dyes, or other molecules to DNA/RNA probes for advanced molecular diagnostics and FISH applications.

Basic Information

Item	Detail
Product Name	6-[2-Biotinylamidoethyl]-Dithiopropionamido]-4,8-Diaza-5,7-Diketoundecanoic Acid, Bis-n-Sulfosuccinimidyl Ester Disodium Salt
CAS No.	1044220-56-5
Molecular Formula	C₃₃H₄₈N₈O₁₄S₃ • 2Na
Molecular Weight	Contact for details
Synonyms	Biotin-PEG2-SS-NHS Ester (disodium salt); Sulfo-NHS-SS-Biotin; NHS-SS-Biotin (sulfonated); Biotinylated Crosslinker with cleavable disulfide bond; Water-soluble Biotin NHS Ester; Heterobifunctional Crosslinker (NHS Ester/Disulfide/Biotin); EZ-Link Sulfo-NHS-SS-Biotin (alternative); 6-[(2-Biotinamidoethyl)dithio]nicotinic Acid NHS Ester Disodium Salt (related structure)
EINECS	Contact for details

Quality Control

Our production of this high-value bioconjugation reagent adheres to stringent quality protocols. Every batch is characterized by advanced analytical techniques, including HPLC for purity assessment and mass spectrometry for identity confirmation. We provide comprehensive Certificates of Analysis (COA) detailing purity, functional activity, and absence of critical impurities, ensuring reliability for your sensitive research and development processes.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Activity	Conforms (by conjugation test)
Solubility	Freely soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.