Description

Smad3 Inhibitor, Sis3 CAS NO 1009104-85-1 is a potent and selective small molecule inhibitor of the Smad3 protein, a key intracellular mediator of Transforming Growth Factor-beta (TGF-β) signaling. This compound is essential for researchers and pharmaceutical developers investigating fibrotic diseases, cancer metastasis, and inflammatory pathways where dysregulated TGF-β/Smad3 signaling is implicated. It is primarily utilized in biochemical research, preclinical drug discovery, and as a critical tool compound for validating therapeutic targets in academic, biotechnology, and pharmaceutical R&D laboratories.

Application

• Biomedical Research: Used as a selective chemical probe to study the specific roles of Smad3 in TGF-β signaling cascades and cellular responses.
• Drug Discovery & Target Validation: Serves as a reference compound in high-throughput screening and lead optimization programs targeting fibrotic disorders.
• Oncology Research: Investigated for its potential to inhibit epithelial-mesenchymal transition (EMT), tumor progression, and metastasis in various cancer cell lines and models.
• Fibrosis Studies: Applied in research models of renal, hepatic, pulmonary, and cardiac fibrosis to elucidate mechanisms and evaluate therapeutic strategies.
• Inflammation & Immunology: Used to dissect the role of Smad3 in T-cell differentiation, autoimmune responses, and chronic inflammatory diseases.
• Cell Signaling Pathway Analysis: A key tool for functional genomics and proteomics studies aimed at mapping TGF-β-dependent cellular networks.

Basic Information

Product Name	Smad3 Inhibitor, Sis3
CAS No.	1009104-85-1
Molecular Formula	C₂₀H₁₈N₄O₂
Molecular Weight	346.38 g/mol
Synonyms	SIS3; Smad3 Inhibitor SIS3; (E)-SIS3; 2-(5-Chloro-2-fluorophenyl)-4-[(4-fluorophenyl)methylene]-5-(4-pyridinyl)-1H-imidazole; 2-(5-Chloro-2-fluorophenyl)-5-(4-pyridinyl)-1H-imidazole-4-carboxaldehyde (4-fluorophenyl)hydrazone; Smad3 Signalling Inhibitor; TGF-β/Smad3 Inhibitor; ALK5 Inhibitor (related pathway)
EINECS	Contact for details

Quality Control

Our Smad3 Inhibitor, Sis3 is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and structural confirmation by NMR and mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your compliance and documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Off-white to yellow solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.