Description

Bis-Pe21- Nhs Ester is a specialized, bifunctional N-hydroxysuccinimide (NHS) ester derivative designed for advanced bioconjugation and crosslinking applications. Its core value lies in enabling the efficient, site-specific labeling and crosslinking of biomolecules, such as proteins and peptides, under mild aqueous conditions. This high-purity reagent is essential for researchers and manufacturers in the fields of proteomics, antibody-drug conjugate (ADC) development, diagnostic assay manufacturing, and materials science, where precise and stable covalent linkages are required.

Application

• Antibody-Drug Conjugate (ADC) Linker Chemistry: Serves as a heterobifunctional crosslinker for covalently attaching cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Labeling: Used to conjugate fluorescent dyes, biotin, or other probes to primary amines (-NH2) on biomolecules for detection and imaging.
• Surface Functionalization: Immobilizes proteins or other amine-containing ligands onto NHS-activated surfaces (e.g., biosensor chips, microarray slides, nanoparticles).
• Polymer and Biomaterial Modification: Incorporates bioactive peptides or proteins into hydrogels and polymeric scaffolds for tissue engineering.
• Diagnostic Reagent Manufacturing: Key component in the production of enzyme conjugates and labeled antigens/antibodies for immunoassays (ELISA, lateral flow tests).
• Affinity Chromatography Resin Preparation: Activates agarose or other matrices for the covalent coupling of affinity ligands.

Basic Information

Product Name	Bis-Pe21- Nhs Ester
CAS No.	1008402-79-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Bis-PEG21-NHS Ester; Bis(PEG21)-NHS Crosslinker; NHS-PEG21-NHS; Polyethylene glycol) bis(N-hydroxysuccinimide) ester (n~21); Bifunctional PEG21 NHS ester; PEG21 di(N-succinimidyl) succinate; Heterobifunctional PEG21 crosslinker
EINECS	Contact for details

Quality Control

Our Bis-Pe21- Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and assay, NMR for structural confirmation, and functional group analysis, to ensure it meets the high standards required for sensitive bioconjugation work. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous liquid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Substitution	≥ 90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.