Description

Immunoglobulin G4,Anti-(Human Integrin R4) (Human-Mouse Monoclonal An100226 C4-Chain),Disulfide With Human-Mouse Monoclonal An100226 Light Chain,Dimer is a highly specific monoclonal antibody reagent designed for research and diagnostic applications targeting the human integrin α4β1 (VLA-4) receptor. This engineered protein is critical for advanced immunological studies, cell adhesion research, and the development of targeted therapeutic strategies. It is an essential tool for pharmaceutical R&D, academic research institutions, and biotechnology companies focused on inflammation, autoimmune diseases, and cancer metastasis.

Application

• Immunological Research: Used as a primary or detection antibody in studies investigating the role of VLA-4 in leukocyte trafficking, adhesion, and signaling pathways.
• Flow Cytometry & Cell Sorting: Enables the identification, quantification, and isolation of cell populations expressing the integrin α4β1 receptor.
• Immunohistochemistry (IHC) & Immunofluorescence (IF): Facilitates the visualization and localization of VLA-4 expression in tissue sections and cell cultures.
• Western Blotting: Serves as a specific probe for detecting the integrin α4 subunit in protein lysates under non-reducing conditions to preserve the dimeric structure.
• Drug Discovery & Development: Acts as a reference standard or blocking agent in assays screening for potential therapeutic molecules that modulate VLA-4 activity.
• Diagnostic Assay Development: Utilized in the development of enzyme-linked immunosorbent assays (ELISA) and other diagnostic kits for monitoring disease biomarkers.

Basic Information

Product Name	Immunoglobulin G4,Anti-(Human Integrin R4) (Human-Mouse Monoclonal An100226 C4-Chain),Disulfide With Human-Mouse Monoclonal An100226 Light Chain,Dimer
CAS No.	189261-10-7
Molecular Formula	Contact for details
Molecular Weight	Approx. 150 kDa (Dimer)
Synonyms	Anti-Integrin alpha 4 beta 1 Antibody (AN100226); Anti-VLA-4 Monoclonal Antibody; Anti-CD49d Antibody; Human-Mouse Chimeric IgG4 Anti-Integrin R4; AN100226 mAb; Anti-ITGA4 Antibody; Monoclonal Antibody AN100226; Anti-Human Integrin α4β1 Dimer
EINECS	Contact for details

Quality Control

This research-grade biological reagent is produced under stringent conditions to ensure high specificity, purity, and batch-to-batch consistency. Quality is verified through a series of analytical methods including SDS-PAGE, size-exclusion chromatography (SEC-HPLC), and endotoxin testing. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, biological activity (e.g., binding affinity via ELISA or SPR), and sterility is provided with each shipment to support your critical research and development work.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted aliquots should be stored at 2-8°C for short-term use or at -70°C for extended storage. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Purity (SDS-PAGE)	> 95%
Protein Concentration	1 mg/mL ± 10% (or as specified per lot)
Endotoxin Level	< 1.0 EU/mg (LAL test)
Biological Activity	Confirmed by ELISA/Flow Cytometry
Buffer (if in solution)	PBS, pH 7.2 - 7.4
Preservative	None (0.09% Sodium Azide may be added)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.