Description

Ala-Ala-Phe-Chloromethylketone Tfa is a specialized tripeptide chloromethyl ketone derivative, a potent and irreversible inhibitor of serine proteases. This compound is critical for biochemical research and pharmaceutical development, offering precise control over enzymatic activity. It is primarily utilized by researchers in proteomics, enzymology, and medicinal chemistry for studying enzyme mechanisms and developing targeted therapeutic agents.

Application

• Protease Inhibition Studies: Acts as a specific, irreversible inhibitor for characterizing serine protease activity and kinetics.
• Biochemical Research: A key tool in enzymology for investigating substrate specificity and catalytic mechanisms.
• Pharmaceutical R&D: Used in the development and screening of novel therapeutic agents targeting protease-related pathways.
• Proteomics: Employed in activity-based protein profiling (ABPP) to identify and validate active serine proteases in complex biological samples.
• Cell Biology: Utilized to modulate specific protease activity in cellular and tissue models to study their physiological roles.
• Reference Standard: Serves as a high-purity analytical standard for quality control and method development in analytical laboratories.

Basic Information

Product Name	Ala-Ala-Phe-Chloromethylketone Tfa
CAS No.	184901-82-4
Molecular Formula	C20H25ClF3N3O6
Molecular Weight	495.88 g/mol
Synonyms	L-Alanyl-L-alanyl-L-phenylalanyl chloromethyl ketone trifluoroacetate; AAPCK; Ala-Ala-Phe-CMK TFA; N-[(Benzyloxy)carbonyl]-L-alanyl-L-alanyl-N-[(1S)-1-(chloromethyl)-3-phenyl-2-oxopropyl]-L-phenylalaninamide TFA; Z-Ala-Ala-Phe-chloromethyl ketone TFA; Chloromethyl ketone inhibitor Z-AAPF-CMK TFA; Protease Inhibitor AAPCK; TFA salt of Ala-Ala-Phe-chloromethyl ketone
EINECS	Contact for details

Quality Control

Our Ala-Ala-Phe-Chloromethylketone Tfa is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture content. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.