Description

Calpain Inhibitor XII is a potent, cell-permeable, and irreversible inhibitor of the calcium-dependent cysteine proteases calpain I and II. This compound is a critical research tool for elucidating the role of calpain activity in cellular processes such as apoptosis, signal transduction, and cytoskeletal remodeling. It is essential for researchers in pharmaceutical development, neuroscience, and cardiovascular disease studies, providing a reliable means to investigate pathological mechanisms and potential therapeutic targets.

Application

• Biomedical Research: A key reagent for studying calpain-mediated pathways in models of neurodegenerative diseases (e.g., Alzheimer's, Parkinson's), muscular dystrophy, and ischemia-reperfusion injury.
• Drug Discovery & Screening: Used as a reference standard and pharmacological tool in high-throughput screening assays to identify and validate novel calpain inhibitors.
• Cell Biology Studies: Employed to inhibit calpain activity to investigate its role in cell migration, proliferation, and apoptosis, particularly in cancer research.
• Neuroscience: Applied in experimental models to understand calpain's involvement in synaptic plasticity, long-term potentiation (LTP), and neuronal cell death following traumatic brain injury or stroke.
• Biochemical Assay Development: Serves as a positive control in enzymatic assays to measure calpain activity and inhibitor potency.

Basic Information

Product Name	Calpain Inhibitor XII
CAS No.	181769-57-3
Molecular Formula	C₂₀H₃₀N₄O₄
Molecular Weight	390.48 g/mol
Synonyms	MDL 28170; Z-Val-Phe-CHO; Carbobenzoxy-Val-Phe-H; Calpain Inhibitor III; (S)-2-(((S)-1-((Benzyloxy)carbonyl)-2-methylpropyl)amino)-1-((S)-3-methyl-1-oxo-1-(phenylamino)butan-2-yl)-2-oxoethyl carbamate; Z-VF-CHO; N-[(Benzyloxy)carbonyl]-L-valyl-N-[(1S)-1-[[(benzyloxy)carbonyl]amino]-3-methyl-1-oxobutan-2-yl]-L-phenylalaninamide
EINECS	Contact for details

Quality Control

Our Calpain Inhibitor XII is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and potency. We adhere to ISO 9001 standards, and our quality protocols are designed to meet the rigorous demands of the global life sciences research community.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Biological Activity (IC₅₀)	≤ 10 nM (Calpain I, in vitro)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.