Description

5-Bromo-2,4-Pyrimidinedione-13C,15N2 is a high-purity, isotopically labeled derivative of a key pyrimidine heterocycle, essential for advanced analytical and research applications. This compound is specifically designed for use as an internal standard in mass spectrometry, enabling precise quantification and metabolic pathway tracing in complex biological matrices. It is a critical reagent for pharmaceutical R&D, particularly in drug metabolism and pharmacokinetics (DMPK) studies, as well as in proteomics and biomarker discovery.

Application

• Mass Spectrometry Internal Standard: Used for the accurate quantification of unlabeled 5-bromo-2,4-pyrimidinedione and related metabolites in LC-MS/MS and GC-MS analyses.
• Drug Metabolism Studies (DMPK): Enables precise tracking of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Proteomics & Biomarker Research: Serves as a stable isotope-labeled precursor or spike-in standard for quantitative proteomic analysis and the discovery of disease biomarkers.
• Nucleic Acid Research: Acts as a labeled building block for studying modified nucleosides, nucleotide analogs, and their biochemical interactions.
• Pharmaceutical Impurity Profiling: Employed in the identification and quantification of process-related impurities and degradation products in active pharmaceutical ingredients (APIs).
• Environmental & Food Safety Testing: Facilitates high-sensitivity detection and quantification of pyrimidine-based compounds in environmental samples and food products.

Basic Information

Product Name	5-Bromo-2,4-Pyrimidinedione-13C,15N2
CAS No.	181517-13-5
Molecular Formula	C313C1H3BrN15N1O2
Molecular Weight	Contact for details
Synonyms	5-Bromouracil-13C,15N2; 5-Bromo-2,4-dihydroxypyrimidine-13C,15N2; 5-Bromo-1H-pyrimidine-2,4-dione-13C,15N2; 5-BrU-13C,15N2; BU-13C,15N2; Isotopically Labeled 5-Bromouracil; [13C,15N2]-5-Bromouracil
EINECS	Contact for details

Quality Control

Every batch of 5-Bromo-2,4-Pyrimidinedione-13C,15N2 is manufactured and tested under strict quality management systems. We guarantee high chemical and isotopic purity to meet the exacting demands of analytical research. Comprehensive testing includes NMR (1H, 13C), mass spectrometry, and HPLC to verify identity, purity, and isotopic enrichment. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting all critical specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C; ≥ 99 atom % 15N
Chemical Purity	≥ 95% (by 1H NMR)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.