Description

Isovaleryl-Phe-Lys-Pna Hcl is a synthetic peptide derivative, specifically a chromogenic substrate designed for high-precision enzymatic assays. This compound is critical for biochemical research and diagnostic development, offering a reliable and sensitive means to detect and quantify specific protease activity. It is primarily utilized by research institutions, pharmaceutical developers, and diagnostic kit manufacturers focused on enzyme kinetics and inhibitor screening.

Application

• Protease Activity Assays: As a chromogenic substrate for the detection and kinetic analysis of specific proteolytic enzymes, including trypsin and related serine proteases.
• Biochemical Research: Used in fundamental research to study enzyme mechanisms, substrate specificity, and inhibition profiles in vitro.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Diagnostic Development: Serves as a key component in the development of clinical diagnostic tests that measure protease levels as biomarkers for various diseases.
• Quality Control in Bioprocessing: Used to monitor and validate the activity of enzyme preparations used in biomanufacturing processes.

Basic Information

Product Name	Isovaleryl-Phe-Lys-Pna Hcl
CAS No.	178894-43-4
Molecular Formula	C27H37ClN6O5
Molecular Weight	561.08 g/mol
Synonyms	Isovaleryl-Phenylalanyl-Lysyl-p-Nitroanilide Hydrochloride; N-Isobutyryl-L-phenylalanyl-N6-[(phenylmethoxy)carbonyl]-L-lysine 4-nitroanilide hydrochloride; IVFK-pNA HCl; 3-Methylbutanoyl-Phe-Lys-pNA HCl; Chromogenic Substrate for Trypsin; Protease Substrate 178894-43-4; Boc-Phe-Lys-pNA (related analog)
EINECS	Contact for details

Quality Control

Our Isovaleryl-Phe-Lys-Pna Hcl is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional activity validation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, identity, and assay results.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Assay (Functional)	≥ 95% of theoretical activity
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.