Description

Arp2/3 Complex Inhibitor I, Inactive Control, Ck-689 is a well-characterized, cell-permeable small molecule specifically designed as a negative control for the active Arp2/3 complex inhibitor CK-666. This product is essential for validating the specificity of actin nucleation inhibition in cellular and biochemical assays. It is a critical research tool for scientists in cell biology, neuroscience, and cancer research who are investigating cytoskeletal dynamics, cell motility, and intracellular trafficking pathways.

Application

• Specificity Control in Cytoskeletal Studies: Serves as a crucial negative control to distinguish the specific effects of Arp2/3 complex inhibition from non-specific or off-target effects of the active inhibitor CK-666.
• Cell Migration and Motility Research: Used in experiments studying lamellipodia formation, cell spreading, and directional migration in cancer metastasis and wound healing models.
• Neuroscience Research: Applied in studies of dendritic spine morphology, synaptic plasticity, and neuronal growth cone dynamics where actin remodeling is key.
• Intracellular Trafficking Assays: Employed to validate the role of Arp2/3 in vesicle transport, endocytosis, and phagocytosis pathways.
• Biochemical Mechanism Validation: Used in in vitro pyrene-actin polymerization assays to confirm that inhibition is due to specific Arp2/3 targeting.
• High-Content Screening (HCS): Acts as a reference compound in phenotypic screening campaigns targeting the actin cytoskeleton.

Basic Information

Product Name	Arp2/3 Complex Inhibitor I, Inactive Control, Ck-689
CAS No.	170930-46-8
Molecular Formula	C₁₄H₁₈N₂O₃
Molecular Weight	262.31 g/mol
Synonyms	CK-689; Arp2/3 Inhibitor I Inactive Control; N-[2-[[4-(4-Morpholinyl)-3-(trifluoromethyl)phenyl]amino]-2-oxoethyl]-3-pyridinecarboxamide; Calbiochem 250225; CAS 170930-46-8; Arp2/3 Complex Negative Control; Inactive analog of CK-666
EINECS	Contact for details

Quality Control

Every batch of our Arp2/3 Complex Inhibitor I, Inactive Control, Ck-689 is manufactured and tested to ensure the highest standards of identity and purity for research applications. Our products undergo rigorous quality testing, including HPLC, NMR, and mass spectrometry analysis, to ensure compliance with stringent in-house specifications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and batch-specific data is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C under desiccant. This compound is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.