Description

γ-Secretase Inhibitor XII is a potent, cell-permeable chemical compound designed to selectively inhibit the γ-secretase enzyme complex. This targeted inhibition is crucial for researchers investigating the Notch signaling pathway and its role in cellular differentiation, proliferation, and apoptosis. It serves as an essential biochemical tool for academic, pharmaceutical, and biotechnology laboratories focused on neurodegenerative disease research, oncology, and developmental biology studies. The compound enables precise modulation of proteolytic processing for advanced in vitro and in vivo applications.

Application

• Notch Signaling Pathway Research: Fundamental tool for studying the role of γ-secretase in Notch receptor cleavage and subsequent transcriptional activation.
• Alzheimer's Disease Models: Used to inhibit the production of amyloid-beta peptides from amyloid precursor protein (APP) in cellular and animal models of neurodegeneration.
• Cancer Biology Studies: Applied in oncology research to investigate the role of Notch signaling in tumor cell proliferation, survival, and angiogenesis.
• Stem Cell & Developmental Biology: Employed to manipulate cell fate decisions and differentiation processes regulated by γ-secretase substrates.
• High-Throughput Screening (HTS): Serves as a reference compound or positive control in drug discovery campaigns targeting the γ-secretase complex.
• Biochemical Mechanism Studies: Utilized in enzyme kinetics and inhibitor profiling assays to understand substrate specificity and inhibitor potency.

Basic Information

Product Name	γ-Secretase Inhibitor XII
CAS No.	161710-10-7
Molecular Formula	C₂₃H₂₂F₂N₄O₃
Molecular Weight	440.44 g/mol
Synonyms	(S)-N-[5-(3,5-Difluorophenyl)-1-oxo-4-(2-propyn-1-yl)-2(S)-hydroxypentyl]-L-leucine tert-butyl ester; DAPT (alternative inhibitor class reference); GS Inhibitor XII; γ-Secretase Inhibitor 12; (2S)-2-{[(1S)-5-(3,5-Difluorophenyl)-1-hydroxy-1-oxopentan-2-yl]amino}-N-[(2S)-1-hydroxy-3-methylbutan-2-yl]-4-methylpentanamide tert-butyl ester; Notch Inhibitor XII; L-685,458 (structurally related compound).
EINECS	Contact for details

Quality Control

Every batch of γ-Secretase Inhibitor XII is manufactured and analyzed under strict quality management protocols to ensure identity, purity, and biological activity. Our products undergo rigorous quality testing to ensure compliance with industry standards for research chemicals. Comprehensive analytical data, including HPLC purity, NMR, and MS confirmation, is documented. Certificates of Analysis (COA) are available upon request to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the product to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. Keep under inert atmosphere for prolonged storage.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M+H]+
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.5%
Biological Activity (Cell-Based Assay)	IC₅₀ consistent with literature range

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.