Description

Z-Vad-Fmk, Non-Methylated is a potent, cell-permeable, and irreversible pan-caspase inhibitor widely used in biochemical and pharmacological research. This non-methylated variant is particularly valuable for studies requiring high specificity and minimal off-target effects in apoptosis signaling pathways. It is an essential research tool for scientists in the fields of oncology, neurobiology, and immunology investigating programmed cell death mechanisms.

Application

• Apoptosis Research: Fundamental tool for inhibiting caspase activity to study the role of programmed cell death in various disease models.
• Oncology Studies: Used to investigate the mechanisms of cancer cell survival and resistance to chemotherapeutic agents.
• Neurodegenerative Disease Research: Applied in models of Alzheimer's, Parkinson's, and stroke to understand neuronal cell death pathways.
• Immunology and Inflammation: Employed to dissect the role of caspases in immune cell regulation and inflammatory responses.
• Drug Discovery & Screening: Serves as a reference compound and positive control in high-throughput assays for novel therapeutic agents targeting apoptosis.
• Cell Culture & Biochemistry: Used to prevent apoptosis in primary cell cultures and to prepare cell lysates for studying caspase substrates.

Basic Information

Product Name	Z-Vad-Fmk, Non-Methylated
CAS No.	161401-82-7
Molecular Formula	C22H31FN4O6
Molecular Weight	466.50 g/mol
Synonyms	Z-VAD(OMe)-FMK; Z-Val-Ala-DL-Asp(OMe)-fluoromethylketone; Benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethyl ketone; Caspase Inhibitor Z-VAD-FMK; Z-VAD-FMK (Non-Methylated); Benzyloxycarbonylvalylalanylaspartic acid fluoromethyl ketone; Pan-Caspase Inhibitor Z-VAD-FMK; (2S)-2-[[(2S)-2-[[(2S)-3-Methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]propanoyl]amino]-4-fluoro-4-oxobutanoic acid methyl ester
EINECS	Contact for details

Quality Control

Our Z-Vad-Fmk, Non-Methylated is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability data. We adhere to relevant quality standards for research-grade biochemicals to support the integrity of your scientific work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (Mass Spectrometry)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.