Description

5-Methyl-[1',2',3',4',5'-13C5]Uridine is a stable isotope-labeled nucleoside analog, specifically a 13C5-labeled derivative of 5-methyluridine. This compound is a critical tool in life science research, enabling precise metabolic tracing and quantification in complex biological systems. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, metabolomics, and stable isotope labeling studies to investigate RNA metabolism, epigenetic modifications, and cellular pathways.

Application

• Metabolic Pathway Tracing: As an internal standard in LC-MS and GC-MS analyses for accurate quantification of nucleoside metabolites.
• Pharmaceutical Research: Used in the development and testing of antiviral and anticancer agents that target RNA synthesis and modification.
• Epigenetics Studies: Employed to study the role of RNA methylation (m5C) in gene expression regulation and disease mechanisms.
• Biochemical Assay Development: Serves as a high-purity reference standard for calibrating analytical instruments and validating testing methods.
• Stable Isotope Labeling: Key precursor for the synthesis of 13C-labeled RNA molecules for NMR-based structural and dynamic studies.
• Diagnostic Kit Manufacturing: Incorporated as a critical raw material in kits designed for the detection of specific RNA modifications.

Basic Information

Product Name	5-Methyl-[1',2',3',4',5'-13C5]Uridine
CAS No.	159496-17-0
Molecular Formula	C1013C5H14N2O6
Molecular Weight	~267.20 g/mol (for 13C5 labeled form)
Synonyms	5-Methyluridine-13C5; 5-Methyl-[13C5]uridine; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methyl-1,2,3,4-tetrahydro-2,4-dioxopyrimidine-13C5; m5U-13C5; Ribothymidine-13C5; 5-Methyl-1-β-D-ribofuranosyluracil-13C5; 5-Methyl-Uridine-13C5
EINECS	Contact for details

Quality Control

Every batch of 5-Methyl-[1',2',3',4',5'-13C5]Uridine is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure the highest standards of isotopic purity, chemical purity, and structural identity. Our quality protocols are designed to meet the stringent requirements of research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (e.g., 13C5 ≥ 99 atom %), chemical purity (by HPLC), and spectroscopic confirmation is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive). For long-term storage, consider storing under an inert atmosphere or in a desiccator to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (MS)	≥ 99 atom % 13C5
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.