Description

5-Bromo-Paps is a high-purity biochemical reagent widely used as a chromogenic substrate for the detection and quantification of alkaline phosphatase activity. This compound is valued for its sensitivity and specificity in enzymatic assays, making it a critical component in diagnostic and research applications. It is primarily utilized by professionals in the pharmaceutical, biotechnology, and clinical diagnostics industries for developing immunoassays, ELISA kits, and other analytical procedures.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): A key chromogenic substrate for detecting alkaline phosphatase conjugates in various ELISA formats.
• Clinical Diagnostics: Used in the development of diagnostic kits for detecting antibodies, antigens, and hormones in patient samples.
• Biochemical Research: Essential for studying enzyme kinetics, inhibition, and activity of alkaline phosphatase in laboratory research.
• Immunohistochemistry (IHC): Employed as a substrate for visualizing alkaline phosphatase-tagged antibodies in tissue sections.
• Western Blotting: Facilitates the detection of proteins via alkaline phosphatase-conjugated secondary antibodies.
• Pharmaceutical Quality Control: Applied in assays to monitor enzymatic activity during drug development and manufacturing processes.

Basic Information

Product Name	5-Bromo-Paps
CAS No.	158905-17-0
Molecular Formula	C14H14BrN2O6PS
Molecular Weight	449.21 g/mol
Synonyms	5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt; 5-Bromo-6-chloro-3-indolyl p-toluidinium phosphate; BCIP p-toluidine salt; 5-Bromo-4-chloro-3-indolyl phosphate toluidine salt (common alternate); BCIP phosphate; 5-Bromo-6-chloro-1H-indol-3-yl dihydrogen phosphate compound with 4-methylbenzenamine; Alkaline Phosphatase Substrate (BCIP); 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
EINECS	Contact for details

Quality Control

Our 5-Bromo-Paps is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. The storage area should be well-ventilated and away from incompatible materials.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Functional Performance	Suitable for ELISA applications

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.